State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, People's Republic of China.
Int J Biol Macromol. 2021 Jul 31;183:90-100. doi: 10.1016/j.ijbiomac.2021.04.077. Epub 2021 Apr 16.
A water-soluble polysaccharide identified here as ADP80-2 was acquired from Angelica dahurica. ADP80-2 was a gluco-arabinan composed of arabinose and a trace of glucose with a molecular weight of 9950 g/mol. The backbone of ADP80-2 comprised →5)-α-L-Araf-(1→, →3, 5)-α-L-Araf-(1→, →6)-α-D-Glcp-(1→, with a terminal branch α-L-Araf-(1 → residue. In terms of immunoregulatory activity, ADP80-2 can significantly promote the phagocytosis, the production of nitric oxide (NO), and the secretion of cytokines (IL-6, IL-1β, and TNF-α) of macrophage. In addition to the cellular immunomodulatory activities, the chemokines related to immunoregulation were significantly increased in the zebrafish model after treated with ADP80-2. These biological results indicated that ADP80-2 with immunomodulatory effects was expected to be useful for the development of new immunomodulatory agents. Simultaneously, the discovery of ADP80-2 further revealed the chemical composition of A. dahurica used as a traditional Chinese medicine and spice.
一种水溶性多糖 ADP80-2 从白芷中获得。ADP80-2 是一种由阿拉伯糖和微量葡萄糖组成的葡甘聚糖，分子量为 9950g/mol。ADP80-2 的主链由→5）-α-L-Araf-（1→、→3、5）-α-L-Araf-（1→、→6）-α-D-Glcp-（1→组成，带有一个末端分支α-L-Araf-（1 →残基。在免疫调节活性方面，ADP80-2 可显著促进巨噬细胞的吞噬作用、一氧化氮（NO）的产生和细胞因子（IL-6、IL-1β和 TNF-α）的分泌。除了细胞免疫调节活性外，ADP80-2 处理后的斑马鱼模型中与免疫调节相关的趋化因子显著增加。这些生物学结果表明，具有免疫调节作用的 ADP80-2 有望成为开发新型免疫调节剂的有用药物。同时，ADP80-2 的发现进一步揭示了白芷作为传统中药和香料的化学成分。
