Constituents from leaves of .

OBJECTIVE

To study constituents of the leaves of , and evaluate their inhibitory effects against NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome activation, and antiproliferative activity.

METHODS

The constituents were isolated and purified by column chromatography on MCI gel CHP20P/P120, silica gel, Sephadex LH-20, and HPLC. The structures of compounds were determined by 1D, 2D NMR, and HR-ESI-MS data. The inhibitory effect of compounds on inflammasome activation was determined by lactate dehydrogenase (LDH) procedure. The antiproliferative activity was evaluated using MTT assay.

RESULTS

The study led to the isolation of 23 compounds, including one new compound, identified as (2)-3-[4-(--glucopyranosyloxy)-2'-hydroxy-5'-methoxyphenyl]-2-propenoic acid (), together with 22 known compounds recognized as 1,4-dihydro-4-oxo-3-pyridinecarbonitrile (), methyl 4-methoxynicotinate (), 4-methoxynicotinonitrile (), 1-(3----glucopyranosyl-4,5-dihydroxyphenyl)-ethanone (), neoisoastilbin (), isoastilbin (), aromadendrin ), neoastilbin (), astilbin (), quercitrin (), neoschaftoside (), apigenin 6,8-bis----arabinoside (), vitexin (), bergenin (), scopoletin (), glucopyranoside salicyl (), koaburside (), benzyl --glucoside (), icariside B5 (), roseoside (), loliolide (), and adenosine (). The tested compounds did not show LDH inhibition nor antiproliferative activity.

CONCLUSION

Compound was a new glycoside. Compounds and were obtained for the first time from natural source. The 22 known compounds constituted of alkaloids (-, ), phenolics (, , , ), flavonoids (-), coumarin (), benzyl glycoside (), and norsesquiterpenes (-). All the compounds, -, were revealed from for the first time. This is the initial uncovering of molecules -, , , -, and from the genus . The isolated compounds, , -, and - established taxonomic classification of in Euphorbiaceae family. Flavonoids were outstanding as chemosystematic markers of genus.