Wang Mei, Monaco Marcia H, Hauser Jonas, Yan Jian, Dilger Ryan N, Donovan Sharon M
Department of Food Science & Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
Société des Produits Nestlé SA, 1000 Lausanne, Switzerland.
Microorganisms. 2021 Apr 21;9(5):884. doi: 10.3390/microorganisms9050884.
Milk oligosaccharides (OS) shape microbiome structure and function, but their relative abundances differ between species. Herein, the impact of the human milk oligosaccharides (HMO) (2'-fucosyllactose [2'FL] and lacto-N-neotetraose [LNnT]) and OS isolated from bovine milk (BMOS) on microbiota composition and volatile fatty acid (VFA) concentrations in ascending colon (AC) contents and feces was assessed. Intact male piglets received diets either containing 6.5 g/L BMOS (n = 12), 1.0 g/L 2'FL + 0.5 g/L LNnT (HMO; n = 12), both (HMO + BMOS; n = 10), or neither (CON; n = 10) from postnatal day (PND) 2 to 34. Microbiota were assessed by 16S rRNA gene sequencing and real-time PCR, and VFA were measured by gas chromatography. The microbiota was affected by OS in an intestine region-specific manner. BMOS reduced ( < 0.05) microbial richness in the AC, microbiota composition in the AC and feces, and acetate concentrations in AC, regardless of HMO presence. HMO alone did not affect overall microbial composition, but increased ( < 0.05) the relative proportion of specific taxa, including , compared to other groups. abundance was increased ( < 0.05) in the AC by BMOS and synergistically by BMOS + HMO in the feces. Distinct effects of HMO and BMOS suggest complementary and sometimes synergistic benefits of supplementing a complex mixture of OS to formula.
乳寡糖（OS）塑造微生物群的结构和功能，但其相对丰度在不同物种间存在差异。在此，评估了人乳寡糖（HMO）（2'-岩藻糖基乳糖[2'FL]和乳糖-N-新四糖[LNnT]）以及从牛乳中分离出的OS（BMOS）对升结肠（AC）内容物和粪便中微生物群组成及挥发性脂肪酸（VFA）浓度的影响。从出生后第2天到第34天，完整的雄性仔猪接受含有6.5 g/L BMOS（n = 12）、1.0 g/L 2'FL + 0.5 g/L LNnT（HMO；n = 12）、两者都含（HMO + BMOS；n = 10）或两者都不含（CON；n = 10）的日粮。通过16S rRNA基因测序和实时PCR评估微生物群，通过气相色谱法测量VFA。微生物群受到OS的影响，且具有肠道区域特异性。无论是否存在HMO，BMOS均降低了（< 0.05）AC中的微生物丰富度、AC和粪便中的微生物群组成以及AC中的乙酸盐浓度。单独的HMO不影响整体微生物组成，但与其他组相比，增加了（< 0.05）特定分类群的相对比例，包括 。BMOS使AC中的 丰度增加（< 0.05），而BMOS + HMO在粪便中具有协同增加作用。HMO和BMOS的不同作用表明，在配方奶中添加复杂的OS混合物具有互补且有时协同的益处。
