罗塞氏菌来源的金属纳米粒子的生物合成及其抗真菌毒素活性。

Biosynthesis and Anti-Mycotoxigenic Activity of Roscoe-Derived Metal Nanoparticles.

机构信息

Department of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia.

Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.

出版信息

Molecules. 2021 Apr 15;26(8):2290. doi: 10.3390/molecules26082290.

Mycotoxigenic fungi have attracted special attention due to their threat to food security and toxicity to human health. Aqueous extract of Roscoe was used as reducing and capping agent for the synthesis of silver (AgNPs), copper (CuNPs), and zinc oxide (ZnONPs) nanoparticles. UV-Visible spectra of the AgNPs, CuNPs, and ZnONPs showed absorption peaks at λ 416 nm, 472 nm, and 372 nm, respectively. Zeta potential of AgNPs, CuNPs, and ZnONPs were -30.9, -30.4 and -18.4 mV, respectively. ZnONPs showed the highest activity against ZUJQ 965830.1 (ZOI 20.9 mm and MIC 24.7 µg/mL). TEM micrographs of ZnONPs-treated showed cracks and pits in the cell wall, liquefaction of the cytoplasmic content, making it less electron-dense. The sporulation and ochratoxin A production of was inhibited by ZnONPs in a concentration-dependent pattern. The inhibition percentage of OTA were 45.6, 84.78 and 95.65% for 10, 15, 20 of ZnONPs/mL, respectively.

由于真菌毒素对食品安全和人类健康的毒性威胁，产毒真菌受到了特别关注。罗克西的水提取物被用作还原和封端剂，用于合成银（AgNPs）、铜（CuNPs）和氧化锌（ZnONPs）纳米粒子。AgNPs、CuNPs 和 ZnONPs 的紫外可见光谱分别在 λ 416nm、472nm 和 372nm 处显示出吸收峰。AgNPs、CuNPs 和 ZnONPs 的zeta 电位分别为-30.9、-30.4 和-18.4mV。ZnONPs 对 ZUJQ 965830.1（ZOI 20.9mm 和 MIC 24.7μg/mL）的活性最高。ZnONPs 处理后的 TEM 显微照片显示细胞壁出现裂缝和凹坑，细胞质内容物液化，使其电子密度降低。ZnONPs 以浓度依赖的方式抑制了的孢子形成和赭曲霉毒素 A 的产生。ZnONPs/mL 分别为 10、15 和 20 时，OTA 的抑制率分别为 45.6%、84.78%和 95.65%。