Newman M J, Light B A, Weston A, Tollurud D, Clark J L, Mann D L, Blackmon J P, Harris C C
Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
J Clin Invest. 1988 Jul;82(1):145-53. doi: 10.1172/JCI113563.
The presence of serum antibodies to the diol-epoxide DNA adducts of representative polycyclic aromatic hydrocarbons (PAH), chrysene, benz[a]anthracene and benzo[a]pyrene, was determined by ELISA using serum samples obtained from normal healthy individuals. Antibodies that reacted against PAH adducted-DNA, but not against PAH-adducted protein, were found in the serum of approximately 40% of the test individuals. Specificity analysis of the antibodies demonstrated that serological cross-reactions between the benzo[a]pyrene and the chrysene diol-epoxide adducts were present. Similar cross-reactivity between the benz[a]anthracene and the chrysene adducts was observed. Sera containing antibodies that were apparently specific for each of the three PAH-DNA adducts were also identified. The presence of antibodies to PAH-DNA adducts indicates both past exposure to these carcinogenic PAH and their metabolic activation to the DNA damaging metabolites. These antibodies may prove to be useful in both retrospective and prospective epidemiological studies of various diseases associated with PAH exposure.
使用从正常健康个体获取的血清样本，通过酶联免疫吸附测定（ELISA）法测定了血清中针对代表性多环芳烃（PAH）、屈、苯并[a]蒽和苯并[a]芘的二醇环氧化物DNA加合物的抗体。在约40%的受试个体血清中发现了与PAH加合DNA反应但不与PAH加合蛋白反应的抗体。抗体的特异性分析表明，苯并[a]芘和屈二醇环氧化物加合物之间存在血清学交叉反应。在苯并[a]蒽和屈加合物之间也观察到类似的交叉反应。还鉴定出了分别对三种PAH-DNA加合物具有明显特异性抗体的血清。PAH-DNA加合物抗体的存在表明过去接触过这些致癌性PAH，以及它们代谢活化为具有DNA损伤作用的代谢产物。这些抗体可能在与PAH暴露相关的各种疾病的回顾性和前瞻性流行病学研究中都有用。
