El-Sheshtawy Sahar M, El-Zoghby Amal F, Shawky Nesreen A, Samak Dalia H
Toxicology Animal Health Research Institute, Tanta Branch, Agriculture Research Centre, Giza, Egypt.
Animal Health Research Institute, Tanta and Zagazig Branch, Agriculture Research Centre, Giza, Egypt.
Vet World. 2021 Mar;14(3):788-793. doi: 10.14202/vetworld.2021.788-793. Epub 2021 Mar 29.
Aflatoxins (AFs) are potent toxic metabolites produced from species. Whose existence in poultry ration leads to drastic economic losses, notably in duck, as the most susceptible poultry species. This study aimed to determine tissue residues of AFs, alterations in selected clinical chemistry variables in serum, mainly during the exposure period, and lycopene and silymarin's possible roles as herbal treatments against aflatoxicosis in Pekin duckling.
The study used one hundred and twenty one-day-old Pekin ducklings and classified them into four groups comprising 30 ducklings in each group. The control group (G1) ducklings were fed a mycotoxin-free ration, and G2 received a naturally contaminated ration with 30 ppb of AFs. G3 and G4 consumed contaminated rations with AFs with 30 ppb for 2 weeks and were treated with lycopene 100 mg/kg or silymarin 600 mg/kg/food, respectively, for 10 days. Serum activities of alanine transaminase and alkaline phosphatase (ALP), glutamyl transferase, ALP, total protein and albumin creatinine and uric acid concentrations, oxidant/antioxidant parameters (malondialdehyde [MDA], total antioxidant capacity (TAC), glutathione S-transferase (GST), and catalase [CAT]), and hepatic AFs residue were determined. Lycopene and silymarin were used for the treatment of aflatoxicosis for another 10 days.
Hepatic and kidney parameters were elevated in the AFs intoxicated group and reduced in the lycopene- and silymarin-treated groups. They had elevated MDA and AFs residues with decreased antioxidant parameters (TAC, GST, and CAT) in the AFs group. At the same time, treatment with lycopene or silymarin had reversed the action of AFs on MDA, elevated the hepatic residue, and improved antioxidant activity.
Lycopene and silymarin, with their potent antioxidant activity, can be used to reverse the harmful effects of AFs on hepatic and kidney tissue.
黄曲霉毒素（AFs）是由某些物种产生的强效有毒代谢产物。其存在于家禽饲料中会导致巨大的经济损失，尤其是在鸭这种最易感的家禽品种中。本研究旨在测定黄曲霉毒素在组织中的残留量、血清中选定临床化学变量的变化（主要是在暴露期），以及番茄红素和水飞蓟宾作为草药治疗北京鸭黄曲霉毒素中毒的可能作用。
本研究使用了120只1日龄的北京鸭，并将它们分为四组，每组30只。对照组（G1）的鸭喂食不含霉菌毒素的饲料，G2组喂食天然污染的含有30 ppb黄曲霉毒素的饲料。G3组和G4组食用含有30 ppb黄曲霉毒素的污染饲料2周，然后分别用100 mg/kg番茄红素或600 mg/kg水飞蓟宾/食物处理10天。测定血清中丙氨酸转氨酶和碱性磷酸酶（ALP）、谷氨酰转移酶、ALP、总蛋白和白蛋白、肌酐和尿酸浓度、氧化/抗氧化参数（丙二醛[MDA]、总抗氧化能力[TAC]、谷胱甘肽S-转移酶[GST]和过氧化氢酶[CAT]）以及肝脏中的黄曲霉毒素残留量。番茄红素和水飞蓟宾再用于治疗黄曲霉毒素中毒10天。
黄曲霉毒素中毒组的肝脏和肾脏参数升高，而番茄红素和水飞蓟宾治疗组的这些参数降低。在黄曲霉毒素组中，它们的MDA和黄曲霉毒素残留量升高，抗氧化参数（TAC、GST和CAT）降低。同时，用番茄红素或水飞蓟宾治疗可逆转黄曲霉毒素对MDA的作用，提高肝脏残留量，并改善抗氧化活性。
番茄红素和水飞蓟宾具有强大的抗氧化活性，可用于逆转黄曲霉毒素对肝脏和肾脏组织的有害影响。
