Abu Bakar Nurul 'Ain, Hakim Abdullah Muhammad Nazrul, Lim Vuanghao, Yong Yoke Keong
Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia.
Evid Based Complement Alternat Med. 2021 Apr 22;2021:5525584. doi: 10.1155/2021/5525584. eCollection 2021.
(MC) is popular for its medicinal uses especially for treating diabetic-related complications. However, the antiulcer activity of essential oil derived from the seeds has not been systematically studied. This study aims to evaluate the gastroprotective activities of essential oil derived from the seed of MC induced by hydrochloride acid/ethanol (HCl/EtOH) and indomethacin and pylorus-ligation model. Gastric ulceration was induced by oral administration of HCl/EtOH solution or indomethacin on day 7 after animals have been pretreated with testing compounds. The first group received just distilled water and the second group received ranitidine (100 mg/kg). Groups 3, 4, and 5 received 10, 50, and 100 mg/kg of essential oil based on their body weight (10 mL/kg), respectively. Macroscopically, pretreatment of essential oil extracted from MC significantly decreased ulceration induced by HCl/EtOH and indomethacin . Microscopically, essential oil also significantly suppressed the formation of edema, epithelial disruption, and mucosa erosions. Moreover, essential oil significantly elevated the pH without decreasing the total acidity of the gastric juice and was able to increase the amount of adherent mucus compared to control. Current results provide scientific basis to the ethno-pharmacological usage of the MC in preventing ulcer formation induced by HCl/EtOH and indomethacin.
葫芦巴因其药用价值而广受欢迎，尤其是在治疗糖尿病相关并发症方面。然而，葫芦巴种子精油的抗溃疡活性尚未得到系统研究。本研究旨在评估葫芦巴种子精油对盐酸/乙醇（HCl/EtOH）和吲哚美辛诱导的胃溃疡以及幽门结扎模型的胃保护作用。在动物用受试化合物预处理7天后，通过口服HCl/EtOH溶液或吲哚美辛诱导胃溃疡。第一组仅接受蒸馏水，第二组接受雷尼替丁（100 mg/kg）。第3、4和5组分别根据体重（10 mL/kg）接受10、50和100 mg/kg的精油。宏观上，葫芦巴提取的精油预处理显著减少了HCl/EtOH和吲哚美辛诱导的溃疡。微观上，精油还显著抑制了水肿、上皮破坏和黏膜糜烂的形成。此外，与对照组相比，精油显著提高了胃液pH值，而不降低总酸度，并且能够增加黏附黏液的量。目前的结果为葫芦巴在预防HCl/EtOH和吲哚美辛诱导的溃疡形成方面的民族药理学应用提供了科学依据。
