Archard L C, Bowles N E, Behan P O, Bell E J, Doyle D
Department of Biochemistry, Charing Cross and Westminster Medical School, London.
J R Soc Med. 1988 Jun;81(6):326-9. doi: 10.1177/014107688808100608.
Enterovirus-specific probes have been prepared by reverse transcription of conserved sequences in purified Coxsackie B2 virus genomic RNA and molecular cloning techniques. These probes were used in quantitative slot blot hybridizations to test for the presence of enterovirus-specific RNA in skeletal muscle biopsy specimens from 96 patients who had suffered from the postviral fatigue syndrome myalgic encephalomyelitis for up to 20 years. Biopsy specimens from 20 patients were positive for the presence of virus-specific RNA with hybridization signals more than three standard deviations greater than the mean of the normal muscle controls. Biopsies from the remaining 76 patients were indistinguishable from the controls. These data show that enterovirus RNA is present in skeletal muscle of some patients with postviral fatigue syndrome up to 20 years after onset of disease and suggest that a persistent virus infection has an aetiological role.
通过对纯化的柯萨奇B2病毒基因组RNA中的保守序列进行逆转录以及分子克隆技术，制备了肠道病毒特异性探针。这些探针被用于定量斑点杂交，以检测96例患有病毒后疲劳综合征——肌痛性脑脊髓炎长达20年的患者的骨骼肌活检标本中肠道病毒特异性RNA的存在情况。20例患者的活检标本病毒特异性RNA呈阳性，杂交信号比正常肌肉对照的平均值高出三个以上标准差。其余76例患者的活检结果与对照组无差异。这些数据表明，在一些病毒后疲劳综合征患者的骨骼肌中，疾病发作后长达20年仍存在肠道病毒RNA，提示持续性病毒感染具有病因学作用。
