The Department of Surgery, The First Dongguan Affiliated Hospital of Guangdong Medical University, Guangdong 523710, China.
The Department of Gastrointestinal Surgery, The Centeral Hospital of Jiangmen, Guangdong 529030, China.
Biomed Res Int. 2021 May 11;2021:5528982. doi: 10.1155/2021/5528982. eCollection 2021.
This study was for verifying that transfecting colon cancer cells (CCCs) with lncRNA NEAT1 packed with siRNA chitosan nanoparticles (CNPs) can suppress lncRNA NEAT1 and biological behaviors of the cells. siRNA targeting lncRNA NEAT1 expression vector was constructed and then transfected into CCCs after being packed with CNPs. Subsequently, the impact of the transfection on biological behaviors of the cells was evaluated. As a result, with high expression in CCCs, NEAT1 was negatively bound up with miR-377-3p in cases with colon cancer (CC), and dual luciferase reporter assay confirmed the potential binding region. Additionally, after downregulating NEAT1 in CCCs, transfection of NEAT1 siRNA packed with CNPs brought a great inhibition on cell proliferation and a promotion on apoptosis, and inhibiting miR-377-3p was able to offset the role of silencing NEAT1 in CCCs. Therefore, in our opinion, NEAT1 siRNA packed with CNPs can hinder the growth and metastasis of CCCs by knocking down NEAT1 in CC, and its mechanism may be achieved by targeting miR-377-3p, which offers a novel direction for treating CC.
本研究旨在验证转染带 siRNA 壳聚糖纳米粒（CNP）的长链非编码 RNA（lncRNA）NEAT1 可抑制 lncRNA NEAT1 和细胞的生物学行为。构建靶向 lncRNA NEAT1 表达载体，并转染至带 CNPs 的结肠癌细胞（CCCs）中。然后，评估转染对细胞生物学行为的影响。结果表明，在结肠癌（CC）中，NEAT1 在 CCCs 中高表达，与 miR-377-3p 负相关，双荧光素酶报告基因实验证实了潜在的结合区域。此外，下调 CCCs 中的 NEAT1 后，转染带 CNP 的 NEAT1 siRNA 可显著抑制细胞增殖并促进细胞凋亡，抑制 miR-377-3p 可抵消沉默 NEAT1 对 CCCs 的作用。因此，我们认为，带 CNP 的 NEAT1 siRNA 通过敲低 CC 中的 NEAT1 可抑制 CCCs 的生长和转移，其机制可能是通过靶向 miR-377-3p 实现的，为治疗 CC 提供了新的方向。
