Babich Olga, Sukhikh Stanislav, Pungin Artem, Astahova Lidiya, Chupakhin Evgeny, Belova Daria, Prosekov Alexander, Ivanova Svetlana
Institute of Living Systems, Immanuel Kant Baltic Federal University, A. Nevskogo Street 14, 236016 Kaliningrad, Russia.
Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, 650043 Kemerovo, Russia.
Plants (Basel). 2021 May 2;10(5):915. doi: 10.3390/plants10050915.
The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of were chosen for study. Nutrient media of various compositions were used for callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.
以愈伤组织培养形式进行植物培养构成了次生植物代谢产物的可再生来源。愈伤组织培养的条件会影响目标化合物的产量。选择了[植物名称]的愈伤组织培养物进行研究。使用了各种成分的营养培养基进行愈伤组织培养。对于每种培养物，获得了关于皂苷、黄酮类化合物和多酚类化合物的定量含量以及抗氧化活性的数据。结果发现，添加了1-萘乙酸和激动素的Murashige和Skoog培养基导致次生代谢产物的产量最高。
