Kamal Rabie, Kharbach Mourad, Heyden Yvan Vander, Yu Huiwen, Bouklouze Abdelaziz, Cherrah Yahia, Alaoui Katim
Pharmacodynamic Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat BP 6203, Rabat Instituts, Rabat 10100, Morocco.
Clinical Research Management (MBA), IU-International University of Applied Sciences, Kaiserplatz 1, 83435 Bad Reichenhall, Germany.
Foods. 2021 May 13;10(5):1078. doi: 10.3390/foods10051078.
The Argan tree (. L) is an evergreen tree endemic of southwestern Morocco. For centuries, various formulations have been used to treat several illnesses including diabetes. However, scientific results supporting these actions are needed. Hence, Argan fruit products (i.e., cake byproducts (saponins extract) and hand pressed Argan oil) were tested for their in-vitro anti-hyperglycemic activity, using α-glucosidase and α-amylase assays. The in-vivo anti-hyperglycemic activity was evaluated in a model of alloxan-induced diabetic mice. The diabetic animals were orally administered 100 mg/kg body weight of aqueous saponins cake extract and 3 mL/kg of Argan oil, respectively, to evaluate the anti-hyperglycemic effect. The blood glucose concentration and body weight of the experimental animals were monitored for 30 days. The chemical properties and composition of the Argan oil were assessed including acidity, peroxides, K232, K270, fatty acids, sterols, tocopherols, total polyphenols, and phenolic compounds. The saponins cake extract produced a significant reduction in blood glucose concentration in diabetic mice, which was better than the Argan oil. This decrease was equivalent to that detected in mice treated with metformin after 2-4 weeks. Moreover, the saponins cake extract showed a strong inhibitory action on α-amylase and α-glucosidase, which is also higher than that of Argan oil.
阿甘树（. L）是摩洛哥西南部特有的一种常绿树。几个世纪以来，人们使用了各种配方来治疗包括糖尿病在内的多种疾病。然而，需要有支持这些作用的科学成果。因此，利用α-葡萄糖苷酶和α-淀粉酶测定法，对阿甘果产品（即饼粕副产品（皂苷提取物）和冷榨阿甘油）的体外抗高血糖活性进行了测试。在四氧嘧啶诱导的糖尿病小鼠模型中评估了其体内抗高血糖活性。分别给糖尿病动物口服100 mg/kg体重的皂苷饼粕水提取物和3 mL/kg的阿甘油，以评估抗高血糖效果。对实验动物的血糖浓度和体重进行了30天的监测。评估了阿甘油的化学性质和成分，包括酸度、过氧化物、K232、K270、脂肪酸、甾醇、生育酚、总多酚和酚类化合物。皂苷饼粕提取物使糖尿病小鼠的血糖浓度显著降低，效果优于阿甘油。这种降低程度与用二甲双胍治疗2 - 4周后的小鼠所检测到的相当。此外，皂苷饼粕提取物对α-淀粉酶和α-葡萄糖苷酶表现出强烈的抑制作用，也高于阿甘油。
