Department of Clinical Science, University of Bergen, 5020 Bergen, Norway.
Proteomics Facility of the University of Bergen (PROBE), University of Bergen, 5020 Bergen, Norway.
Int J Mol Sci. 2021 May 26;22(11):5665. doi: 10.3390/ijms22115665.
Mesenchymal stem cells (MSCs) can differentiate into osteoblasts, and therapeutic targeting of these cells is considered both for malignant and non-malignant diseases. We analyzed global proteomic profiles for osteoblasts derived from ten and MSCs from six healthy individuals, and we quantified 5465 proteins for the osteoblasts and 5420 proteins for the MSCs. There was a large overlap in the profiles for the two cell types; 156 proteins were quantified only in osteoblasts and 111 proteins only for the MSCs. The osteoblast-specific proteins included several extracellular matrix proteins and a network including 27 proteins that influence intracellular signaling (Wnt/Notch/Bone morphogenic protein pathways) and bone mineralization. The osteoblasts and MSCs showed only minor age- and sex-dependent proteomic differences. Finally, the osteoblast and MSC proteomic profiles were altered by ex vivo culture in serum-free media. We conclude that although the proteomic profiles of osteoblasts and MSCs show many similarities, we identified several osteoblast-specific extracellular matrix proteins and an osteoblast-specific intracellular signaling network. Therapeutic targeting of these proteins will possibly have minor effects on MSCs. Furthermore, the use of ex vivo cultured osteoblasts/MSCs in clinical medicine will require careful standardization of the ex vivo handling of the cells.
间充质干细胞（MSCs）可以分化为成骨细胞，因此针对这些细胞的治疗方法被认为可用于治疗恶性和非恶性疾病。我们分析了源自 10 位健康个体的成骨细胞和源自 6 位健康个体的间充质干细胞的全球蛋白质组谱，并对成骨细胞定量了 5465 种蛋白质，对间充质干细胞定量了 5420 种蛋白质。两种细胞类型的蛋白质组谱有很大的重叠；156 种蛋白质仅在成骨细胞中被定量，111 种蛋白质仅在间充质干细胞中被定量。成骨细胞特异性蛋白质包括几种细胞外基质蛋白质和一个包含 27 种蛋白质的网络，这些蛋白质影响细胞内信号（Wnt/Notch/骨形态发生蛋白途径）和骨矿化。成骨细胞和间充质干细胞仅显示出轻微的年龄和性别依赖性蛋白质组差异。最后，在无血清培养基中外体培养改变了成骨细胞和间充质干细胞的蛋白质组谱。我们得出结论，尽管成骨细胞和间充质干细胞的蛋白质组谱显示出许多相似之处，但我们鉴定出了几种成骨细胞特异性细胞外基质蛋白质和一个成骨细胞特异性细胞内信号网络。针对这些蛋白质的治疗方法可能对间充质干细胞的影响较小。此外，在临床医学中使用体外培养的成骨细胞/间充质干细胞将需要仔细标准化细胞的体外处理。
