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患有和未患呼吸道合胞病毒细支气管炎的婴儿的鼻腔和粪便微生物群及免疫分析

Nasal and Fecal Microbiota and Immunoprofiling of Infants With and Without RSV Bronchiolitis.

作者信息

Alba Claudio, Aparicio Marina, González-Martínez Felipe, González-Sánchez María Isabel, Pérez-Moreno Jimena, Toledo Del Castillo Blanca, Rodríguez Juan Miguel, Rodríguez-Fernández Rosa, Fernández Leonides

机构信息

Department of Nutrition and Food Science, Complutense University of Madrid, Madrid, Spain.

Department of Pediatrics, Hospital Universitario Gregorio Marañón, Madrid, Spain.

出版信息

Front Microbiol. 2021 Jun 1;12:667832. doi: 10.3389/fmicb.2021.667832. eCollection 2021.

DOI:10.3389/fmicb.2021.667832
PMID:34140944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8203809/
Abstract

Bronchiolitis associated with the respiratory syncytial virus (RSV) is the leading cause of hospitalization among infants aged < 1 year. The main objective of this work was to assess the nasal and fecal microbiota and immune profiles in infants with RSV bronchiolitis, and to compare them with those of healthy infants. For this purpose, a total of 58 infants with RSV-positive bronchiolitis and 17 healthy infants (aged < 18 months) were recruited in this case-control study, which was approved by the Ethics Committee of the Hospital Gregorio Marañón. Nasal and fecal samples were obtained and submitted to bacterial microbiota analysis by 16S rDNA sequencing and to analysis of several immune factors related to inflammatory processes. Nasal samples in which and/or accounted for > 20% of the total sequences were exclusively detected among infants of the bronchiolitis group. In this group, the relative abundances of and were significantly lower than in nasal samples from the control group while the opposite was observed for those of and . Fecal bacterial microbiota of infants with bronchiolitis was similar to that of healthy infants. Significant differences were obtained between bronchiolitis and control groups for both the frequency of detection and concentration of BAFF/TNFSF13B and sTNF.R1 in nasal samples. The concentration of BAFF/TNFSF13B was also significantly higher in fecal samples from the bronchiolitis group. In conclusion, signatures of RSV-associated bronchiolitis have been found in this study, including dominance of and a high concentration of BAFF/TNFSF13B, IL-8 and sTNF.R1 in nasal samples, and a high fecal concentration of BAFF/TNFSF13B.

摘要

呼吸道合胞病毒（RSV）相关的细支气管炎是1岁以下婴儿住院的主要原因。这项工作的主要目的是评估RSV细支气管炎婴儿的鼻腔和粪便微生物群及免疫谱，并将其与健康婴儿的进行比较。为此，在这项病例对照研究中，共招募了58例RSV阳性细支气管炎婴儿和17例健康婴儿（年龄<18个月），该研究获得了格雷戈里奥·马拉尼翁医院伦理委员会的批准。采集鼻腔和粪便样本，通过16S rDNA测序进行细菌微生物群分析，并分析与炎症过程相关的几种免疫因子。仅在细支气管炎组婴儿中检测到鼻腔样本中 和/或 占总序列的>20%。在该组中， 和 的相对丰度显著低于对照组的鼻腔样本，而 和 的相对丰度则相反。细支气管炎婴儿的粪便细菌微生物群与健康婴儿相似。在鼻腔样本中，细支气管炎组和对照组在BAFF/TNFSF13B和sTNF.R1的检测频率和浓度方面均存在显著差异。细支气管炎组粪便样本中BAFF/TNFSF13B的浓度也显著更高。总之，本研究发现了RSV相关细支气管炎的特征，包括鼻腔样本中 占优势以及BAFF/TNFSF13B、IL-8和sTNF.R1浓度高，以及粪便中BAFF/TNFSF13B浓度高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1ca/8203809/975cf8697aa1/fmicb-12-667832-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b1ca/8203809/975cf8697aa1/fmicb-12-667832-g007.jpg

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3
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