光流体流通式生物传感器灵敏度——模型与实验
Optofluidic Flow-Through Biosensor Sensitivity - Model and Experiment.
作者信息
Wright Joel G, Amin Md Nafiz, Meena Gopikrishnan G, Schmidt Holger, Hawkins Aaron R
机构信息
Department of Electrical and Computer Engineering, Brigham Young University, Provo, UT, 84602, USA.
School of Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA.
出版信息
J Lightwave Technol. 2021 May 15;39(10):3330-3340. doi: 10.1109/jlt.2021.3061872. Epub 2021 Feb 24.
We present a model and simulation for predicting the detected signal of a fluorescence-based optical biosensor built from optofluidic waveguides. Typical applications include flow experiments to determine pathogen concentrations in a biological sample after tagging relevant DNA or RNA sequences. An overview of the biosensor geometry and fabrication processes is presented. The basis for the predictive model is also outlined. The model is then compared to experimental results for three different biosensor designs. The model is shown to have similar signal statistics as physical tests, illustrating utility as a pre-fabrication design tool and as a predictor of detection sensitivity.
我们提出了一个模型和模拟方法，用于预测基于光流体波导构建的荧光光学生物传感器的检测信号。典型应用包括在标记相关DNA或RNA序列后，通过流动实验测定生物样品中的病原体浓度。本文介绍了生物传感器的几何结构和制造工艺概况，概述了预测模型的基础。然后将该模型与三种不同生物传感器设计的实验结果进行比较。结果表明，该模型具有与物理测试相似的信号统计特性，说明了其作为预制设计工具和检测灵敏度预测器的实用性。
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