Craniomaxillofacial Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Asian Pac J Cancer Prev. 2021 Jul 1;22(7):2185-2189. doi: 10.31557/APJCP.2021.22.7.2185.
MicroRNAs (miRs) are a group of endogenous, non-coding, 18-24 nucleotide length single-strand RNAs. These molecules mediate the gene expression and are involved in regulating diverse cellular biological processes, i.e. cell cycle, differentiation, and apoptosis. Aberrant miR expression has been shown to be an important event in the pathologies of various types of cancer, including oral squamous cell carcinoma (OSCC).
Blood samples were obtained from 30 patients (15 cases and 15 controls), to determine miR-138 and miR-424-5p expression by using real-time PCR and ΔΔCT.
The median CT values of miR-138 were 27.60 and 28.70, while those of miR 424-5p were 29.40 and 30.0 in the case and control groups, respectively. Mann-Whitney test indicated no significant difference in miR-138 and miR-424-5p between the two groups (P > 0.05). However, results obtained by ΔΔCT method showed that miR-424-5p expression was 1.96 times higher in the case group, but miR-138 expression was 3.05 times lower in the plasma of OSCC patients.
Our findings suggest that the evaluation of miR-138 and miR-424-5p expression in serum can be used as potent markers for carcinoma detection and also may be a potentially therapeutic approach in the future. Further longitudinal studies with larger samples are required to verify these findings.
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microRNAs（miRs）是一组内源性、非编码、18-24 个核苷酸长度的单链 RNA。这些分子介导基因表达，并参与调节多种细胞生物学过程，如细胞周期、分化和凋亡。异常的 miR 表达已被证明是各种类型癌症（包括口腔鳞状细胞癌[OSCC]）病理的重要事件。
从 30 名患者（15 例和 15 例对照）中获得血液样本，通过实时 PCR 和ΔΔCT 来确定 miR-138 和 miR-424-5p 的表达。
miR-138 的中位 CT 值分别为 27.60 和 28.70，miR-424-5p 的中位 CT 值分别为 29.40 和 30.0，在病例组和对照组中。Mann-Whitney 检验表明 miR-138 和 miR-424-5p 在两组之间无显著差异（P>0.05）。然而，通过ΔΔCT 方法得到的结果显示，miR-424-5p 在病例组中的表达高出 1.96 倍，而 miR-138 在 OSCC 患者血浆中的表达低 3.05 倍。
我们的研究结果表明，血清中 miR-138 和 miR-424-5p 的表达评估可作为癌检测的有效标志物，并且可能是未来潜在的治疗方法。需要进一步进行样本量大的纵向研究来验证这些发现。
