Department of Neurology, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neurotrauma, Neurorepair, and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin, China.
Front Cell Infect Microbiol. 2021 Jul 13;11:644448. doi: 10.3389/fcimb.2021.644448. eCollection 2021.
Gut microbiota dysbiosis following stroke affects the recovery of neurological function. Administration of prebiotics to counteract post-stroke dysbiosis may be a potential therapeutic strategy to improve neurological function. We aim to observe the effect of lactulose on neurological function outcomes, gut microbiota composition, and plasma metabolites in mice after stroke.
Male C57BL/6 mice (20-25 g) were randomly divided into three groups: healthy control, photothrombotic stroke + triple-distilled water, and photothrombotic stroke + lactulose. After 14 consecutive days of lactulose administration, feces, plasma, and organs were collected. 16S rDNA sequencing, plasma untargeted metabolomics, qPCR, flow cytometry and Elisa were performed.
Lactulose supplementation significantly improved the functional outcome of stroke, downregulated inflammatory reaction, and increased anti-inflammatory factors in both the brain and gut. In addition, lactulose supplementation repaired intestinal barrier injury, improved gut microbiota dysbiosis, and partially amended metabolic disorder after stroke.
Lactulose promotes functional outcomes after stroke in mice, which may be attributable to repressing harmful bacteria, and metabolic disorder, repairing gut barrier disruption, and reducing inflammatory reactions after stroke.
中风后肠道微生物失调会影响神经功能的恢复。使用益生元来对抗中风后失调可能是改善神经功能的一种潜在治疗策略。我们旨在观察乳果糖对中风后小鼠神经功能结局、肠道微生物组成和血浆代谢物的影响。
雄性 C57BL/6 小鼠（20-25g）随机分为三组：健康对照组、光血栓性中风+三蒸水组和光血栓性中风+乳果糖组。连续 14 天给予乳果糖后，收集粪便、血浆和器官。进行 16S rDNA 测序、血浆非靶向代谢组学、qPCR、流式细胞术和 Elisa。
乳果糖补充显著改善了中风后的功能结局，下调了大脑和肠道中的炎症反应，并增加了抗炎因子。此外，乳果糖补充修复了肠道屏障损伤，改善了中风后的肠道微生物失调，并部分纠正了代谢紊乱。
乳果糖促进了中风后小鼠的功能结局，这可能归因于抑制有害细菌和代谢紊乱，修复肠道屏障破坏，并减少中风后的炎症反应。
