Kwon Haeun, Nguyen Quynh Nhu, Na Myung Woo, Kim Ki Hyun, Guo Yuanqiang, Yim Joung Han, Shim Sang Hee, Kim Jae-Jin, Kang Ki Sung, Lee Dongho
Department of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
College of Korean Medicine, Gachon University, Seongnam, Republic of Korea.
J Antibiot (Tokyo). 2021 Dec;74(12):893-897. doi: 10.1038/s41429-021-00473-8. Epub 2021 Sep 8.
A new α-pyrone analog, arthrifuranone A (1) was isolated from an EtOAc-extract of Arthrinium pseudosinense culture medium. The isolation workflow was guided by a Molecular Networking-based dereplication strategy. The chemical structure of the new compound was elucidated using MS and NMR spectroscopic techniques, and the absolute configuration was established by the Mosher's method and gauge-including atomic orbital NMR chemical shift calculations, followed by DP4 + analysis. The isolated compound was evaluated for its estrogenic activity using the MCF-7 estrogen responsive human breast cancer cells. Compound 1 showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 3.125 μM via phosphorylation of estrogen receptor-α.
从拟中华节菱孢培养基的乙酸乙酯提取物中分离出一种新的α-吡喃酮类似物——关节炎呋喃酮A（1）。分离工作流程以基于分子网络的去重复策略为指导。使用质谱和核磁共振光谱技术阐明了新化合物的化学结构，并通过莫舍尔法和含规范原子轨道核磁共振化学位移计算确定了绝对构型，随后进行了DP4 +分析。使用MCF-7雌激素反应性人乳腺癌细胞评估了分离出的化合物的雌激素活性。化合物1在浓度为3.125 μM时通过雌激素受体-α的磷酸化增加MCF-7细胞的增殖，从而表现出雌激素活性。
