Jiang Yuyan, Pu Kanyi
School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.
School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
Chem Rev. 2021 Nov 10;121(21):13086-13131. doi: 10.1021/acs.chemrev.1c00506. Epub 2021 Sep 24.
Optical imaging is an indispensable tool in clinical diagnostics and fundamental biomedical research. Autofluorescence-free optical imaging, which eliminates real-time optical excitation to minimize background noise, enables clear visualization of biological architecture and physiopathological events deep within living subjects. Molecular probes especially developed for autofluorescence-free optical imaging have been proven to remarkably improve the imaging sensitivity, penetration depth, target specificity, and multiplexing capability. In this Review, we focus on the advancements of autofluorescence-free molecular probes through the lens of particular molecular or photophysical mechanisms that produce long-lasting luminescence after the cessation of light excitation. The versatile design strategies of these molecular probes are discussed along with a broad range of biological applications. Finally, challenges and perspectives are discussed to further advance the next-generation autofluorescence-free molecular probes for in vivo imaging and in vitro biosensors.
光学成像在临床诊断和基础生物医学研究中是一种不可或缺的工具。无自发荧光光学成像通过消除实时光学激发以最小化背景噪声，能够清晰地可视化活体深部的生物结构和生理病理事件。专门为无自发荧光光学成像开发的分子探针已被证明能显著提高成像灵敏度、穿透深度、靶标特异性和多重检测能力。在本综述中，我们通过特定分子或光物理机制的视角，聚焦于无自发荧光分子探针的进展，这些机制在光激发停止后能产生持久发光。这些分子探针的通用设计策略以及广泛的生物学应用也将被讨论。最后，还将讨论挑战和前景，以进一步推动用于体内成像和体外生物传感器的下一代无自发荧光分子探针的发展。
