Omara Reda Ibrahim, Nehela Yasser, Mabrouk Ola Ibrahim, Elsharkawy Mohsen Mohamed
Wheat Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt.
Department of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31511, Egypt.
Biology (Basel). 2021 Sep 16;10(9):925. doi: 10.3390/biology10090925.
Characterization of the genetic structure and the physiological races of is a growing necessity to apply host genetic resistance against wheat leaf rust as a successful control strategy. Herein, we collected and identified about 130 isolates of from 16 Egyptian commercial wheat cultivars grown at different locations, over two seasons (2019/2020 and 2020/2021). The 130 isolates of were segregated into 17 different physiological races. TTTST and TTTKS were the most common virulent races, whereas TTTST and MTTGT were the most frequent races. The races were classified into three groups, based on their distinct DNA band sizes (150 bp, 200 bp, and 300 bp) after RAPD analysis. The new wheat cultivars (Sakha-94, Sakha-95, and Shandweel-1) infected with the most virulent race (TTTST), Gemmeiza-12, and Misr-3 were resistant to all physiological races. The resistance of these cultivars was mostly due to the presence of and resistant genes. Our results serve as a warning about emerging aggressive races capable of supplanting resistance to leaf rust, and help in the understanding of the pathotype-cultivar-location association and its role in the susceptibility/resistance of new wheat cultivars to
鉴定小麦叶锈菌的遗传结构和生理小种对于将寄主遗传抗性作为一种成功的防治策略来应用愈发必要。在此，我们在两个生长季（2019/2020和2020/2021）从埃及不同地点种植的16个商业小麦品种中收集并鉴定了约130个小麦叶锈菌分离株。这130个小麦叶锈菌分离株被分为17个不同的生理小种。TTTST和TTTKS是最常见的毒性小种，而TTTST和MTTGT是最频繁出现的小种。基于随机扩增多态性DNA（RAPD）分析后不同的DNA条带大小（150 bp、200 bp和300 bp），这些小种被分为三组。感染最具毒性小种（TTTST）的新小麦品种（Sakha - 94、Sakha - 95和Shandweel - 1）、Gemmeiza - 12和Misr - 3对所有生理小种均具有抗性。这些品种的抗性主要归因于Lr和抗性基因的存在。我们的结果警示了可能取代叶锈抗性的新兴侵袭性小种的出现，并有助于理解致病型 - 品种 - 地点关联及其在新小麦品种对叶锈易感性/抗性中的作用
