Department of Biological Systems Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Department of Food Engineering, Dankook University, Cheonan, Chungnam, Republic of Korea.
Compr Rev Food Sci Food Saf. 2021 Nov;20(6):5829-5855. doi: 10.1111/1541-4337.12843. Epub 2021 Oct 2.
Ensuring consistently high quality and safety is paramount to food producers and consumers alike. Wet chemistry and microbiological methods provide accurate results, but those methods are not conducive to rapid, onsite testing needs. Hence, many efforts have focused on rapid testing for food quality and safety, including the development of various biosensors. Herein, we focus on a group of biosensors, which provide visually recognizable colorimetric signals within minutes and can be used onsite. Although there are different ways to achieve visual color-change signals, we restrict our focus on sensors that exploit the localized surface plasmon resonance (LSPR) phenomenon of metal nanoparticles, primarily gold and silver nanoparticles. The typical approach in the design of LSPR biosensors is to conjugate biorecognition ligands on the surface of metal nanoparticles and allow the ligands to specifically recognize and bind the target analyte. This ligand-target binding reaction leads to a change in color of the test sample and a concomitant shift in the ultraviolet-visual absorption peak. Various designs applying this and other signal generation schemes are reviewed with an emphasis on those applied for evaluating factors that compromise the quality and safety of food and agricultural products. The LSPR-based colorimetric biosensing platform is a promising technology for enhancing food quality and safety. Aided by the advances in nanotechnology, this sensing technique lends itself easily for further development on field-deployable platforms such as smartphones for onsite and end-user applications.
确保始终如一的高质量和安全性对食品生产者和消费者来说都是至关重要的。湿法化学和微生物方法提供了准确的结果，但这些方法不利于快速、现场测试的需求。因此，许多人致力于快速检测食品质量和安全性，包括各种生物传感器的开发。在这里，我们专注于一组生物传感器，它们可以在几分钟内提供肉眼可见的比色信号，并可在现场使用。虽然有不同的方法可以实现视觉变色信号，但我们将重点放在利用金属纳米粒子的局域表面等离子体共振（LSPR）现象的传感器上，主要是金和银纳米粒子。在 LSPR 生物传感器的设计中，典型的方法是在金属纳米粒子的表面上连接生物识别配体，并允许配体特异性地识别和结合目标分析物。这种配体-靶结合反应导致测试样品的颜色变化，并伴随着紫外-可见吸收峰的位移。我们回顾了应用这种方法和其他信号产生方案的各种设计，重点介绍了那些应用于评估影响食品和农产品质量和安全的因素的设计。基于 LSPR 的比色生物传感平台是增强食品质量和安全性的一项有前途的技术。借助纳米技术的进步，这种传感技术很容易在现场可部署平台（如智能手机）上进一步开发，用于现场和最终用户应用。
