基于核酸的分子计算正在走向细胞应用。

Nucleic acid-based molecular computation heads towards cellular applications.

机构信息

MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou 350108, P. R. China.

College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, P. R. China.

出版信息

Chem Soc Rev. 2021 Nov 15;50(22):12551-12575. doi: 10.1039/d0cs01508c.

DOI:10.1039/d0cs01508c

PMID:34604889

Abstract

Nucleic acids, with the advantages of programmability and biocompatibility, have been widely used to design different kinds of novel biocomputing devices. Recently, nucleic acid-based molecular computing has shown promise in making the leap from the test tube to the cell. Such molecular computing can perform logic analysis within the confines of the cellular milieu with programmable modulation of biological functions at the molecular level. In this review, we summarize the development of nucleic acid-based biocomputing devices that are rationally designed and chemically synthesized, highlighting the ability of nucleic acid-based molecular computing to achieve cellular applications in sensing, imaging, biomedicine, and bioengineering. Then we discuss the future challenges and opportunities for cellular and applications. We expect this review to inspire innovative work on constructing nucleic acid-based biocomputing to achieve the goal of precisely rewiring, even reconstructing cellular signal networks in a prescribed way.

摘要

核酸具有可编程性和生物相容性的优势，已被广泛用于设计不同类型的新型生物计算设备。最近，基于核酸的分子计算在将试管中的技术推向细胞内的应用方面显示出了巨大的潜力。这种分子计算可以在细胞环境的限制内进行逻辑分析，同时在分子水平上对生物功能进行可编程的调控。在这篇综述中，我们总结了经过合理设计和化学合成的基于核酸的生物计算设备的发展，重点介绍了基于核酸的分子计算在传感、成像、生物医学和生物工程等方面实现细胞应用的能力。然后，我们讨论了该领域在细胞内和活体应用方面所面临的未来挑战和机遇。我们希望这篇综述能够激发人们构建基于核酸的生物计算的创新性工作，从而实现精确地重新布线，甚至以预定的方式重建细胞信号网络的目标。