来自哺乳动物大脑的吗啡和可待因。
Morphine and codeine from mammalian brain.
作者信息
Weitz C J, Lowney L I, Faull K F, Feistner G, Goldstein A
出版信息
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9784-8. doi: 10.1073/pnas.83.24.9784.
Recently, we described the presence of six immunoreactive (ir) morphinans in bovine adrenal and hypothalamus and identified one as morphine [Goldstein, A., Barrett, R. W., James, I. F., Lowney, L. I., Weitz, C. J., Knipmeyer, L. L. & Rapaport, H. (1985) Proc. Natl. Acad. Sci. USA 82, 5203-5207]. We now report that ir morphinans corresponding to the previously reported peak 1 (morphine), peak 4, and peak 5 are consistently present in extracts of bovine hypothalamus and variably present in extracts of bovine adrenal and rat brain. We no longer detect the previously reported peaks 2, 3, or 6, and we have established that they were contamination artifacts. Peak 1 is coeluted with morphine in two distinct reversed-phase HPLC systems, as is peak 4 with codeine. We have purified peak 1 and peak 4 compounds from bovine hypothalamus and determined their identities by gas chromatography/mass spectrometry (GC/MS): peak 1 is confirmed to be morphine and peak 4 is codeine.
最近，我们描述了牛肾上腺和下丘脑中有六种免疫反应性（ir）吗啡喃的存在，并鉴定出其中一种为吗啡[戈尔茨坦，A.，巴雷特，R. W.，詹姆斯，I. F.，洛尼，L. I.，韦茨，C. J.，克尼普迈尔，L. L. & 拉帕波特，H.（1985年）《美国国家科学院院刊》82，5203 - 5207]。我们现在报告，与先前报道的峰1（吗啡）、峰4和峰5相对应的ir吗啡喃始终存在于牛下丘脑提取物中，在牛肾上腺和大鼠脑提取物中则有变化地存在。我们不再检测到先前报道的峰2、峰3或峰6，并且已经确定它们是污染假象。在两种不同的反相高效液相色谱系统中，峰1与吗啡共洗脱，峰4与可待因共洗脱。我们从牛下丘脑纯化了峰1和峰4化合物，并通过气相色谱/质谱（GC/MS）确定了它们的身份：峰1被确认为吗啡，峰4为可待因。
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