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支架蛋白 CcmM 指导β-羧化体生物发生中的多蛋白相分离。

Scaffolding protein CcmM directs multiprotein phase separation in β-carboxysome biogenesis.

机构信息

Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Martinsried, Germany.

出版信息

Nat Struct Mol Biol. 2021 Nov;28(11):909-922. doi: 10.1038/s41594-021-00676-5. Epub 2021 Nov 10.

DOI:10.1038/s41594-021-00676-5
PMID:34759380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8580825/
Abstract

Carboxysomes in cyanobacteria enclose the enzymes Rubisco and carbonic anhydrase to optimize photosynthetic carbon fixation. Understanding carboxysome assembly has implications in agricultural biotechnology. Here we analyzed the role of the scaffolding protein CcmM of the β-cyanobacterium Synechococcus elongatus PCC 7942 in sequestrating the hexadecameric Rubisco and the tetrameric carbonic anhydrase, CcaA. We find that the trimeric CcmM, consisting of γCAL oligomerization domains and linked small subunit-like (SSUL) modules, plays a central role in mediation of pre-carboxysome condensate formation through multivalent, cooperative interactions. The γCAL domains interact with the C-terminal tails of the CcaA subunits and additionally mediate a head-to-head association of CcmM trimers. Interestingly, SSUL modules, besides their known function in recruiting Rubisco, also participate in intermolecular interactions with the γCAL domains, providing further valency for network formation. Our findings reveal the mechanism by which CcmM functions as a central organizer of the pre-carboxysome multiprotein matrix, concentrating the core components Rubisco and CcaA before β-carboxysome shell formation.

摘要

在蓝藻中，羧基体将 Rubisco 和碳酸酐酶等酶包裹起来，以优化光合作用中的碳固定。了解羧基体的组装在农业生物技术中有重要意义。在这里，我们分析了β-蓝藻集胞藻 PCC 7942 的支架蛋白 CcmM 在隔离十六聚体 Rubisco 和四聚体碳酸酐酶 CcaA 中的作用。我们发现，由γCAL 寡聚化结构域和连接的小亚基样 (SSUL) 模块组成的三聚体 CcmM 在通过多价、协同相互作用介导前羧基体凝聚物形成中发挥核心作用。γCAL 结构域与 CcaA 亚基的 C 末端尾部相互作用，此外还介导 CcmM 三聚体的头对头缔合。有趣的是，SSUL 模块除了其在招募 Rubisco 中的已知功能外，还与 γCAL 结构域参与分子间相互作用，为网络形成提供了进一步的价数。我们的研究结果揭示了 CcmM 作为前羧基体多蛋白基质的中央组织者的作用机制，在β-羧基体壳形成之前浓缩核心成分 Rubisco 和 CcaA。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fda9/8580825/c810aab4aeb2/41594_2021_676_Fig13_ESM.jpg
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