Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Key Laboratory of Dairy Products Processing, Ministry of Agriculture, Inner Mongolia Agricultural University, Huhhot, China.
Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
FEMS Microbiol Ecol. 2021 Dec 1;97(12). doi: 10.1093/femsec/fiab151.
Previous studies demonstrated that multi-strain probitics could more strongly regulate intestinal cytokines and the mucosal barrier than the individual ingredient strains. Nevertheless, the potentially different gut microbiome modulation effects between multi-strain and single-strain probiotics treatments remain unexplored. Here, we administered three different Lactiplantibacillus plantarum strains or their mixture to healthy Wistar rats and compared the shift of gut microbiome among the treatment groups. A 4-week intervention with mixed probiotics induced more drastic and diversified gut microbiome modulation than single-strain probiotics administration (alpha diversity increased 8% and beta diversity increased 18.7%). The three single-strain probiotics treatments all converged the gut microbiota, decreasing between-individual beta diversity by 12.7% on average after the treatment, while multi-strain probiotics treatment diversified the gut microbiome and increased between-individual beta diversity by 37.2% on average. Covariation analysis of the gut microbes suggests that multi-strain probiotics could exert synergistic, modified and enhanced modulation effects on the gut microbiome based on strain-specific modulation effects of probiotics. The more heterogeneous responses to the multi-strain probiotics treatment suggest that future precision microbiome modulation should consider the potential interactions of the probiotic strains, and personalized response to probiotic formulas due to heterogenous gut microbial compositions.
先前的研究表明，多菌株益生菌比单一成分菌株更能有效地调节肠道细胞因子和黏膜屏障。然而，多菌株和单菌株益生菌治疗之间可能存在不同的肠道微生物组调节作用，这一点仍有待探索。在这里，我们给健康的 Wistar 大鼠喂食三种不同的植物乳杆菌菌株或它们的混合物，并比较了治疗组之间肠道微生物组的变化。混合益生菌的 4 周干预比单一菌株益生菌给药更能引起更剧烈和多样化的肠道微生物组调节（α多样性增加 8%，β多样性增加 18.7%）。三种单一菌株益生菌处理都使肠道菌群聚集，治疗后个体间β多样性平均减少了 12.7%，而多菌株益生菌处理使肠道微生物组多样化，个体间β多样性平均增加了 37.2%。肠道微生物的共变分析表明，多菌株益生菌可能通过益生菌菌株特有的调节作用，对肠道微生物组发挥协同、改良和增强的调节作用。对多菌株益生菌治疗的反应更加多样化，这表明未来的精准微生物组调节应考虑益生菌菌株的潜在相互作用，以及由于肠道微生物组成的异质性而导致的对益生菌配方的个性化反应。
