Department of Bioprocess Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, ul. Wolczanska 213, 90-924 Lodz, Poland.
Biomolecules. 2021 Nov 22;11(11):1740. doi: 10.3390/biom11111740.
The aim of this study was to quantitatively characterize the morphology of the filamentous microorganisms ATCC 20542 and ATCC 10970, cocultivated in stirred tank bioreactors, and to characterize their mutual influence with the use of quantitative image analysis. Three distinct coculture initiation strategies were applied: preculture versus preculture, spores versus spores and preculture versus preculture with time delay for one of the species. Bioreactor cocultures were accompanied by parallel monoculture controls. The results recorded for the mono- and cocultures were compared in order to investigate the effect of cocultivation on the morphological evolution of and . Morphology-related observations were also confronted with the analysis of secondary metabolism. The morphology of the two studied filamentous species strictly depended on the applied coculture initiation strategy. In the cocultures initiated by the simultaneous inoculation, gained domination or advance over . The latter microorganism dominated only in these experiments in which was introduced with a delay.
本研究的目的是定量描述在搅拌罐生物反应器中共培养的丝状微生物 ATCC 20542 和 ATCC 10970 的形态，并通过定量图像分析来描述它们之间的相互影响。采用了三种不同的共培养起始策略：预培养物对预培养物、孢子对孢子和一种物种的预培养物对预培养物加时间延迟。生物反应器共培养伴随着平行的单一培养对照。对单培养和共培养的记录结果进行了比较，以研究共培养对 和 形态演变的影响。还将与二次代谢分析相对比形态学观察结果。两种研究丝状物种的形态严格依赖于所应用的共培养起始策略。在同时接种启动的共培养中， 获得了优势或超过 的进展。在后一种微生物仅在 延迟引入的实验中占主导地位。
