Mavrommatis Alexandros, Simitzis Panagiotis E, Kyriakaki Panagiota, Giamouri Elisavet, Myrtsi Eleni D, Evergetis Epameinondas, Filippi Katiana, Papapostolou Harris, Koulocheri Sofia D, Pappas Athanasios C, Koutinas Apostolis, Haroutounian Serkos A, Tsiplakou Eleni
Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Laboratory of Animal Breeding & Husbandry, Department of Animal Science, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Animals (Basel). 2021 Oct 22;11(11):3038. doi: 10.3390/ani11113038.
The valorization of vinification byproducts portrays a promising bioprocess for the enrichment of animals' diet with bioactive compounds, such as polyphenols, which could regulate the immune response. Therefore, the impact of dietary grounded grape pomace (GGP), wine lees extract (WYC), and grape stem extract (PE) on the relative transcript level of immune related genes of broiler chickens were examined. Two hundred forty, one-day-old as hatched (male/female) chicks (Ross 308) were allocated to four dietary groups, with four replicate pens each with 15 birds. Birds were fed either a basal diet (CON) or the basal diet supplemented with 2.5% GGP, or 0.2% WYC, or 0.1% PE for 42 d. The relative expression of immune-related genes was investigated using a real-time PCR platform. The mRNA levels of Toll-like Receptor 4 ( were downregulated ( = 0.039) in the liver of broilers fed the GGP-containing diet compared to the CON, while in the spleen of PE-fed broilers, was significantly upregulated ( = 0.043). The mRNA levels of interleukin 8 ( tended to upregulate ( = 0.099) in the bursa of Fabricius and were significantly increased ( = 0.036) in the spleen of broilers fed the PE diet. Vinification byproducts depict a promising sustainable source of polyphenols for the poultry feed industry, but more research is needed under field conditions.
葡萄酒酿造副产物的增值描绘了一种很有前景的生物工艺，可用于在动物饮食中富集生物活性化合物，如多酚，这些化合物可以调节免疫反应。因此，研究了日粮中磨碎的葡萄皮渣（GGP）、酒糟提取物（WYC）和葡萄茎提取物（PE）对肉鸡免疫相关基因相对转录水平的影响。将240只1日龄（雌雄混合）雏鸡（罗斯308）分为四个日粮组，每组四个重复栏，每个栏有15只鸡。给鸡喂食基础日粮（CON）或添加2.5%GGP、0.2%WYC或0.1%PE的基础日粮，持续42天。使用实时PCR平台研究免疫相关基因的相对表达。与CON组相比，喂食含GGP日粮的肉鸡肝脏中Toll样受体4（）的mRNA水平下调（=0.039），而在喂食PE的肉鸡脾脏中， 显著上调（=0.043）。喂食PE日粮的肉鸡法氏囊中白细胞介素8（）的mRNA水平有上调趋势（=0.099），在脾脏中显著升高（=0.036）。葡萄酒酿造副产物为家禽饲料工业描绘了一种很有前景的可持续多酚来源，但在田间条件下还需要更多研究。
