Spinelli Veronica, Ceci Andrea, Dal Bosco Chiara, Gentili Alessandra, Persiani Anna Maria
Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Microorganisms. 2021 Oct 20;9(11):2179. doi: 10.3390/microorganisms9112179.
Glyphosate is the most commonly used herbicide worldwide. Its improper use during recent decades has resulted in glyphosate contamination of soils and waters. Fungal bioremediation is an environmentally friendly, cost effective, and feasible solution to glyphosate contamination in soils. In this study, several saprotrophic fungi isolated from agricultural environments were screened for their ability to tolerate and utilise Roundup in different cultural conditions as a nutritional source. was further screened to evaluate the ability to break down and utilise glyphosate as a P source in a liquid medium. The dose-response effect for Roundup, and the difference in toxicity between pure glyphosate and Roundup were also studied. This study reports the ability of several strains to tolerate 1 mM and 10 mM Roundup and to utilise it as nutritional source. was reported for the first time for its ability to degrade glyphosate to a considerable extent (80%) and to utilise it as a P source, without showing dose-dependent negative effects on growth. Pure glyphosate was found to be more toxic than Roundup for . Our results showed that pure glyphosate toxicity can be only partially addressed by the pH decrease determined in the culture medium. In conclusion, our study emphasises the noteworthy potential of in glyphosate degradation.
草甘膦是全球最常用的除草剂。近几十年来其不当使用导致了土壤和水体的草甘膦污染。真菌生物修复是解决土壤中草甘膦污染的一种环境友好、成本效益高且可行的方法。在本研究中，对从农业环境中分离出的几种腐生真菌进行了筛选，以评估它们在不同培养条件下耐受和利用农达作为营养源的能力。进一步筛选以评估在液体培养基中分解和利用草甘膦作为磷源的能力。还研究了农达的剂量反应效应以及纯草甘膦和农达之间的毒性差异。本研究报告了几种菌株耐受1 mM和10 mM农达并将其用作营养源的能力。首次报道了其在很大程度上（80%）降解草甘膦并将其用作磷源的能力，且对生长未表现出剂量依赖性负面影响。发现纯草甘膦对……的毒性比农达更大。我们的结果表明，培养基中pH值的降低只能部分解决纯草甘膦的毒性问题。总之，我们的研究强调了……在草甘膦降解方面的显著潜力。
