Department of Electricity and Energy, Vocational School of Technical Sciences, Akdeniz University, Antalya, Turkey.
Department of Medical Imaging Techniques, Vocational School of Health Services, Akdeniz University, Antalya, Turkey.
Mikrochim Acta. 2021 Dec 11;189(1):24. doi: 10.1007/s00604-021-05128-x.
A novel molecularly imprinted electrochemical biosensor for glucose detection is reported based on a hierarchical N-rich carbon conductive-coated TNO structure (TNO@NC). Firstly, TNO@NC was fabricated by a novel polypyrrole-chemical vapor deposition (PPy-CVD) method with minimal waste generation. Afterward, the electrode modification with TNO@NC was performed by dropping TNO@NC particles on glassy carbon electrode surfaces by infrared heat lamp. Finally, the glucose-imprinted electrochemical biosensor was developed in presence of 75.0 mM pyrrole and 25.0 mM glucose in a potential range from + 0.20 to + 1.20 V versus Ag/AgCl via cyclic voltammetry (CV). The physicochemical and electrochemical characterizations of the fabricated molecularly imprinted biosensor was conducted by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) method, X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and CV techniques. The findings demonstrated that selective, sensitive, and stable electrochemical signals were proportional to different glucose concentrations, and the sensitivity of molecularly imprinted electrochemical biosensor for glucose detection was estimated to be 18.93 μA μM cm (R = 0.99) at + 0.30 V with the limit of detection (LOD) of 1.0 × 10 M. Hence, it can be speculated that the fabricated glucose-imprinted biosensor may be used in a multitude of areas, including public health and food quality.
一种基于富氮碳涂层 TNO 结构（TNO@NC）的新型分子印迹电化学葡萄糖生物传感器被报道。首先，通过一种新型的聚吡咯-化学气相沉积（PPy-CVD）方法，以最小的废物生成量制备了 TNO@NC。然后，通过红外热灯将 TNO@NC 颗粒滴在玻碳电极表面上进行电极修饰。最后，在 +0.20 至 +1.20 V 相对于 Ag/AgCl 的电位范围内，通过循环伏安法（CV）在存在 75.0 mM 吡咯和 25.0 mM 葡萄糖的情况下开发了葡萄糖印迹电化学生物传感器。通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X 射线衍射（XRD）方法、X 射线光电子能谱（XPS）、电化学阻抗谱（EIS）和 CV 技术对制备的分子印迹生物传感器的物理化学和电化学特性进行了表征。研究结果表明，选择性、灵敏性和稳定的电化学信号与不同的葡萄糖浓度成正比，并且葡萄糖分子印迹电化学生物传感器的灵敏度估计为 18.93 μA μM cm（R = 0.99），在 +0.30 V 时，葡萄糖检测的检测限（LOD）为 1.0×10 M。因此，可以推测制备的葡萄糖印迹生物传感器可能会应用于多个领域，包括公共卫生和食品安全。
