用于研究有丝分裂期间动粒-微管附着的体外和体内方法。
In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis.
作者信息
Afreen Sana, Rahi Amit, Landeros Adriana G, Chakraborty Manas, McKenney Richard J, Varma Dileep
机构信息
Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Department of Molecular and Cellular Biology, University of California-Davis, Davis, CA, USA.
出版信息
Methods Mol Biol. 2022;2415:123-138. doi: 10.1007/978-1-0716-1904-9_9.
The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.
有丝分裂过程中复制染色体的分离是细胞分裂过程中的关键步骤。因此，为确保动粒微管（kMTs）正确附着和稳定而发生的一系列精心编排的事件是有丝分裂领域备受关注的热点。在这里，我们描述了通过体外生化方法和体内细胞生物学方法研究kMT附着的方法。
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