Zielińska Sylwia, Matkowski Adam, Dydak Karolina, Czerwińska Monika Ewa, Dziągwa-Becker Magdalena, Kucharski Mariusz, Wójciak Magdalena, Sowa Ireneusz, Plińska Stanisława, Fijałkowski Karol, Ciecholewska-Juśko Daria, Broda Michał, Gorczyca Damian, Junka Adam
Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biotechnology, Wroclaw Medical University, 50-556 Wroclaw, Poland.
Department of Pharmaceutical Biology and Biotechnology, Division of Pharmaceutical Biology and Botany, Wroclaw Medical University, 50-556 Wroclaw, Poland.
Materials (Basel). 2021 Dec 21;15(1):16. doi: 10.3390/ma15010016.
In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of -a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic bacteria and used in three forms: (1) Without removal of cells, (2) partially cleaned up from the remaining cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against , and . The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing.
在这项工作中，我们开发了一种双功能细菌纳米纤维素（BNC）载体系统，用于培养一种能产生抗菌化合物的药用植物细胞。多孔BNC由非致病细菌生物合成3、5或7天，并以三种形式使用：（1）不除去细胞；（2）用水洗涤部分清除剩余细胞；（3）用NaOH完全纯化，不残留细菌细胞。将悬浮细胞接种在液体培养基中的BNC片上，收获功能化的BNC，进行扫描电子显微镜观察，分析代谢物含量、进行抗菌试验，并检测其在人中性粒细胞中的潜在促炎刺激活性。在未经纯化的3日龄BNC中发现了最高含量和最复杂组成的药理活性物质，并对其生物活性进行了测试。基于体外模型中IL-1β、IL-8和TNF-α分泌的试验显示了这种特定生物基质的抗炎作用。此外，3日龄的BNC对[具体菌种1]、[具体菌种2]和[具体菌种3]具有抗菌和抗生物膜活性。研究结果表明，这种改性复合材料可能用于对抗微生物病原体，特别是在局部表面感染中，富含植物代谢物的BNC可作为封闭敷料使用。
