Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
Center for General Education, Cheng Shiu University, Kaohsiung, Taiwan.
Sci Rep. 2022 Feb 18;12(1):2828. doi: 10.1038/s41598-022-06892-3.
This study aimed to evaluate the effects of the stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) on cartilage injury in an osteoarthritis (OA) rat model. Sodium iodoacetate (3 mg/50 μL) was used to induce OA in the left knee joint of rats. On day 14 after OA induction, 50 μL of SVF (5 × 10cells), ADSCs (1 × 10 cells), or 0.9% normal saline (NS) was injected into the left knee-joint cavity of each group. The macroscopic view and histological sections revealed that the articular cartilage in the NS group was damaged, inflamed, uneven and thin, and had hyperchromatic cell infiltration. Notably, the cartilage surface had recovered to nearly normal and appeared smooth and bright on day 14 in the SVF and ADSC groups. Additionally, the white blood cell counts in the SVF and ADSC groups were higher than those in the NS group on day 14. Plasma IL-1β levels on days 7 and 14 were reduced in the SVF and ADSC groups. These results indicated that both SVF and ADSC treatments may assist in articular cartilage regeneration after cartilage injury. Cell therapy may benefit patients with OA. However, clinical trials with humans are required before the application of SVF and ADSC treatments in patients with OA.
本研究旨在评估基质血管成分（SVF）和脂肪来源干细胞（ADSCs）对骨关节炎（OA）大鼠模型中软骨损伤的影响。采用碘乙酸钠（3mg/50μL）诱导大鼠左膝关节 OA。在 OA 诱导后第 14 天，将 50μL 的 SVF（5×10 个细胞）、ADSCs（1×10 个细胞）或 0.9%生理盐水（NS）注入每组大鼠的左膝关节腔。大体观察和组织学切片显示，NS 组的关节软骨受损、炎症、不均匀且变薄，并有深染细胞浸润。值得注意的是，SVF 和 ADSC 组的软骨表面在第 14 天已恢复接近正常，呈现光滑明亮。此外，SVF 和 ADSC 组在第 14 天的白细胞计数高于 NS 组。SVF 和 ADSC 组的血浆 IL-1β水平在第 7 天和第 14 天降低。这些结果表明，SVF 和 ADSC 治疗可能有助于软骨损伤后的关节软骨再生。细胞治疗可能有益于 OA 患者。然而，在将 SVF 和 ADSC 治疗应用于 OA 患者之前，需要进行人体临床试验。
