Department of Cardiology, Baoquanling Central Hospital, Hegang City, Heilongjiang Province, PR. China.
Bioengineered. 2022 Mar;13(3):6272-6279. doi: 10.1080/21655979.2022.2031395.
It has been reported that circHIPK3 can be downregulated by high glucose, suggesting its potential involvement in diabetes and diabetic complications. This study aimed to explore the role of circHIPK3 in diabetic cardiomyopathy (DC). PTEN is a kind of tumor suppressor gene, which is very commonly lost in human cancer. We detected the expression of circHIPK3 and PTEN in plasma samples from DC patients, diabetic patients without complications diabetes mellitus (DM) and health controls by RT-qPCR and ELISA. In vitro cell experiment, AC16 cells (cardiomyocytes) were treated with high glucose, followed by expression analysis of circHIPK3 and PTEN mRNA by RT-qPCR. CircHIPK3 or PTEN expression vector were used to overexpress circHIPK3 and PTEN in AC16 cells to explore the relationship between them. The role of circHIPK3 and PTEN in regulating the apoptosis of AC16 cells was analyzed by cell apoptosis assay. The result showed that CircHIPK3 was downregulated in diabetes and further downregulated in DC. In AC16 cells, high glucose treatment decreased the expression levels of circHIPK3. Across DC samples, the expression of circHIPK3 was inversely correlated with PTEN. In AC16 cells, overexpression of circHIPK3 decreased the expression levels of PTEN. CircHIPK3 may suppress AC16 cell apoptosis induced by high glucose and inhibited the role of PTEN in cell apoptosis. Therefore, circHIPK3 may downregulate PTEN to protect cardiomyocytes from high glucose-induced cell apoptosis.
已有报道称，circHIPK3 可被高葡萄糖下调，提示其可能参与糖尿病及糖尿病并发症的发生。本研究旨在探讨 circHIPK3 在糖尿病心肌病（DC）中的作用。PTEN 是一种肿瘤抑制基因，在人类癌症中常丢失。我们通过 RT-qPCR 和 ELISA 检测了 DC 患者、无并发症糖尿病（DM）患者和健康对照者血浆样本中 circHIPK3 和 PTEN 的表达。体外细胞实验中，用高葡萄糖处理 AC16 细胞（心肌细胞），然后通过 RT-qPCR 分析 circHIPK3 和 PTEN mRNA 的表达。使用 circHIPK3 或 PTEN 表达载体过表达 AC16 细胞中的 circHIPK3 和 PTEN，以探讨它们之间的关系。通过细胞凋亡实验分析 circHIPK3 和 PTEN 在调节 AC16 细胞凋亡中的作用。结果显示，糖尿病时 CircHIPK3 下调，在 DC 中进一步下调。在 AC16 细胞中，高葡萄糖处理降低 circHIPK3 的表达水平。在 DC 样本中，circHIPK3 的表达与 PTEN 呈负相关。在 AC16 细胞中，circHIPK3 的过表达降低了 PTEN 的表达水平。CircHIPK3 可能抑制高葡萄糖诱导的 AC16 细胞凋亡，并抑制 PTEN 在细胞凋亡中的作用。因此，circHIPK3 可能通过下调 PTEN 来保护心肌细胞免受高葡萄糖诱导的细胞凋亡。
