Department of Biomedical Engineering, Sogang University, Seoul 04107, Korea.
Department of Food and Nutrition, KC University, Seoul 07661, Korea.
Int J Mol Sci. 2022 Feb 17;23(4):2204. doi: 10.3390/ijms23042204.
In this study, we fabricated a three-dimensional (3D) scaffold using industrial polylactic acid (PLA), which promoted the proliferation and differentiation of human neural stem cells. An industrial PLA 3D scaffold (IPTS) cell chip with a square-shaped pattern was fabricated via computer-aided design and printed using a fused deposition modeling technique. To improve cell adhesion and cell differentiation, we coated the IPTS cell chip with gold nanoparticles (Au-NPs), nerve growth factor (NGF) protein, an NGF peptide fragment, and sonic hedgehog (SHH) protein. The proliferation of F3.Olig2 neural stem cells was increased in the IPTS cell chips coated with Au-NPs and NGF peptide fragments when compared with that of the cells cultured on non-coated IPTS cell chips. Cells cultured on the IPTS-SHH cell chip also showed high expression of motor neuron cell-specific markers, such as HB9 and TUJ-1. Therefore, we suggest that the newly engineered industrial PLA scaffold is an innovative tool for cell proliferation and motor neuron differentiation.
在这项研究中，我们使用工业聚乳酸（PLA）制造了一种三维（3D）支架，促进了人神经干细胞的增殖和分化。通过计算机辅助设计制造了具有方形图案的工业 PLA 3D 支架（IPTS）细胞芯片，并使用熔丝制造技术进行打印。为了提高细胞黏附性和细胞分化，我们在 IPTS 细胞芯片上涂覆了金纳米粒子（Au-NPs）、神经生长因子（NGF）蛋白、NGF 肽片段和 sonic hedgehog（SHH）蛋白。与在未涂覆的 IPTS 细胞芯片上培养的细胞相比，涂覆有 Au-NPs 和 NGF 肽片段的 IPTS 细胞芯片上的 F3.Olig2 神经干细胞增殖增加。在 IPTS-SHH 细胞芯片上培养的细胞也表现出运动神经元细胞特异性标志物（如 HB9 和 TUJ-1）的高表达。因此，我们认为新设计的工业 PLA 支架是促进细胞增殖和运动神经元分化的创新工具。
