Lu Yanyi, Zeng Lin, Li Min, Yan Bowen, Gao Dandan, Zhou Bangfu, Lu Weiping, He Qinghua
Department of Military Medical Equipment, State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Army Medical University, 400042, Chongqing, China.
Clinical Laboratory, Daping Hospital, Army Medical University, 400042, Chongqing, China.
AMB Express. 2022 Mar 4;12(1):31. doi: 10.1186/s13568-022-01367-0.
In order to explore the possibility to identify common wound infection bacteria in mixed culture with gas chromatograph-ion migration spectroscopy (GC-IMS), the headspace gas of single and mixed cultures of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were detected and analyzed by GC-IMS system. The bacteria were cultured in thioglycolate medium tubes then transferred to the sampling bottles (indirect method), or directly cultured in the sampling bottles (direct method) to allow accumulation of volatile compounds and facilitate automation. The specific microorganism volatile organic compounds (mVOCs) of the three bacteria were obtained. Some of them have been known to certain substance, for example, ethanol, isoamyl acetate, Phenylacetaldehyde, 2-heptanone etc., while others have not. Principal component analysis (PCA) showed that a higher separability can be achieved with direct method than indirect method. This work indicated that it is possible to identify compound bacteria by detecting specific mVOCs with GC-IMS, and the specific mVOCs should be medium-dependent.
为了探索利用气相色谱-离子迁移谱（GC-IMS）在混合培养物中鉴定常见伤口感染细菌的可能性，采用GC-IMS系统对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的单菌培养物和混合培养物的顶空气体进行了检测和分析。细菌在硫乙醇酸盐培养基管中培养，然后转移到采样瓶中（间接法），或直接在采样瓶中培养（直接法），以积累挥发性化合物并便于自动化操作。获得了这三种细菌的特定微生物挥发性有机化合物（mVOCs）。其中一些已被确认为某些特定物质，例如乙醇、乙酸异戊酯、苯乙醛、2-庚酮等，而其他一些则未知。主成分分析（PCA）表明，直接法比间接法具有更高的可分离性。这项工作表明，通过GC-IMS检测特定的mVOCs来鉴定复合细菌是可行的，并且特定的mVOCs应依赖于培养基。
