Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Oncology, Ganzhou Cancer Hospital, Gannan Medical University,Ganzhou, Jiangxi, China.
Bioengineered. 2022 Mar;13(3):6665-6677. doi: 10.1080/21655979.2022.2045843.
The role of lung microbiota in non-small cell lung cancer remains unclear. We investigated the characteristics and functional roles of lung microbiota in non-small cell lung cancer. Bronchoalveolar lavage fluid samples were obtained from patients with non-small cell lung cancer (n = 46) and with benign lung disease (n = 29). The differences in composition and gene expression in the microbiota between the samples were analyzed using 16s rRNA sequencing. The oncogenic genus () was then evaluated in the progression of lung cancer in C57 BL/6 mice. Compared to benign lung disease, the lung microbiota in non-small cell lung cancer was significantly altered, both in terms of α- and β-diversity. In terms of bacterial composition, the non-small cell lung cancer group was enriched with two Phyla (Firmicutes, Bacteroidetes) and three genera (). and were most strongly associated with non-small cell lung cancer, and significantly promoted the progression of lung cancer . Moreover, metabolic prediction revealed that ribosomes, biosynthesis of secondary metabolites, and pyrimidine metabolism were among the enriched pathways that may be involved in the progression of non-small cell lung cancer. Overall, results suggest that the progression of non-small cell lung cancer is followed by significant changes in the composition and function of the lung microbiota. These differing genera may be potential diagnostic markers and therapeutic targets.
肺微生物组在非小细胞肺癌中的作用尚不清楚。我们研究了肺微生物组在非小细胞肺癌中的特征和功能作用。收集非小细胞肺癌患者（n=46）和良性肺部疾病患者（n=29）的支气管肺泡灌洗液样本。使用 16s rRNA 测序分析样本中微生物组在组成和基因表达上的差异。然后在 C57BL/6 小鼠中评估致癌属（）在肺癌进展中的作用。与良性肺部疾病相比，非小细胞肺癌患者的肺微生物组在 α 和 β 多样性方面均发生了显著改变。在细菌组成方面，非小细胞肺癌组中两个门（厚壁菌门、拟杆菌门）和三个属（）富集。和 与非小细胞肺癌的相关性最强，而 显著促进了肺癌的进展。此外，代谢预测表明，核糖体、次生代谢物的生物合成和嘧啶代谢是可能参与非小细胞肺癌进展的富集途径之一。总的来说，结果表明非小细胞肺癌的进展伴随着肺微生物组组成和功能的显著变化。这些不同的属可能是潜在的诊断标志物和治疗靶点。
