Third Level Research Laboratory of State Administration of Traditional Chinese Medicine, Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, P. R. China.
Institute of Traditional Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, P. R. China.
Food Funct. 2022 Mar 21;13(6):3590-3602. doi: 10.1039/d1fo04089h.
has been used both as a traditional medicine and as a functional food for hundreds of years in Asia. However, the active constituents from and their pharmacologic properties still need to be further explored. In this study, one new dammarane-type triterpenoid saponin (1), along with fourteen known analogs (2-15) were isolated and identified from the roots of . The anti-inflammatory, anti-angiogenetic and anti-dengue virus effects of these isolated compounds were further evaluated. Compounds 1, 3, 5-7 and 10-12 exerted anti-inflammatory effects in two different zebrafish inflammatory models. Among them, 11, with the most significant activities, alleviated the inflammatory response by blocking the MyD88/NF-κB and STAT3 pathways. Moreover, compound 15 showed anti-angiogenetic activities in Tg() and Tg() zebrafish, while 3 and 5 only inhibited angiogenesis in Tg() zebrafish. Additionally, compounds 1, 3, 6, 8, 9 and 12 suppressed the replication of dengue virus either at the viral adsorption and entry stages or at the intracellular replication step. In conclusion, these findings enrich knowledge of the diversity of saponins in and suggest that the dammarane-type triterpenoid saponins from may be developed as potential functional foods to treat inflammation, angiogenesis or dengue-related diseases.
在亚洲，已经有数百年的历史，被用作传统药物和功能性食品。然而，仍需要进一步探索 和 的活性成分及其药理特性。在这项研究中，从 根部分离并鉴定出一种新的达玛烷型三萜皂苷（1），以及 14 种已知类似物（2-15）。进一步评估了这些分离化合物的抗炎、抗血管生成和抗登革热病毒作用。化合物 1、3、5-7 和 10-12 在两种不同的斑马鱼炎症模型中表现出抗炎作用。其中，11 具有最显著的活性，通过阻断 MyD88/NF-κB 和 STAT3 通路来缓解炎症反应。此外，化合物 15 在 Tg()和 Tg()斑马鱼中表现出抗血管生成活性，而 3 和 5 仅在 Tg()斑马鱼中抑制血管生成。此外，化合物 1、3、6、8、9 和 12 抑制登革热病毒的复制，无论是在病毒吸附和进入阶段还是在细胞内复制阶段。总之，这些发现丰富了 中皂苷多样性的知识，并表明 中的达玛烷型三萜皂苷可能被开发为治疗炎症、血管生成或登革热相关疾病的潜在功能性食品。
