Liu Chunlu, Zuo Zhitian, Xu Furong, Wang Yuanzhong
Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, China.
Collge of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan, China.
Front Plant Sci. 2023 Feb 7;13:1009727. doi: 10.3389/fpls.2022.1009727. eCollection 2022.
The cultivation and sale of medicinal plants are some of the main ways to meet the increased market demand for plant-based drugs. is a widely used Chinese medicinal material. The growth and accumulation of bioactive constituents mainly depend on a satisfactory growing environment. Additionally, the occurrence of market fraud means that care should be taken when purchasing.
In this study, we report the correlation between saponins and climate factors based on high performance liquid chromatography (HPLC), and evaluate the influence of climate factors on the quality of . In addition, the synchronous two-dimensional correlation spectroscopy (2D-COS) images of near infrared (NIR) data combined with the deep learning model were applied to traceability of geographic origins of at two different levels (district and town levels).
The results indicated that the contents of saponins in are negatively related to the annual mean temperature and the temperature annual range. A lower annual mean temperature and temperature annual range are favorable for the content accumulation of saponins. Additionally, high annual precipitation and high humidity are conducive to the content accumulation of Notoginsenoside R1 (NG-R1), Ginsenosides Rg1 (G-Rg1), and Ginsenosides Rb1 (G-Rb1), while Ginsenosides Rd (G-Rd), this is not the case. Regarding geographic origins, classifications at two different levels could be successfully distinguished through synchronous 2D-COS images combined with the residual convolutional neural network (ResNet) model. The model accuracy of the training set, test set, and external validation is achieved at 100%, and the cross-entropy loss function curves are lower. This demonstrated the potential feasibility of the proposed method for geographic origin traceability, even if the distance between sampling points is small.
The findings of this study could improve the quality of , provide a reference for cultivating in the future and alleviate the occurrence of market fraud.
药用植物的种植和销售是满足市场对植物性药物不断增长的需求的一些主要方式。三七是一种广泛使用的中药材。生物活性成分的生长和积累主要取决于适宜的生长环境。此外,市场欺诈的出现意味着购买时应谨慎。
在本研究中,我们基于高效液相色谱法(HPLC)报告了皂苷与气候因素之间的相关性,并评估了气候因素对三七质量的影响。此外,将近红外(NIR)数据的同步二维相关光谱(2D-COS)图像与深度学习模型相结合,应用于三七在两个不同层面(区和镇层面)的地理溯源。
结果表明,三七中皂苷的含量与年平均温度和气温年较差呈负相关。较低的年平均温度和气温年较差有利于皂苷含量的积累。此外,高年降水量和高湿度有利于三七皂苷R1(NG-R1)、人参皂苷Rg1(G-Rg1)和人参皂苷Rb1(G-Rb1)的含量积累,而人参皂苷Rd(G-Rd)则不然。关于地理来源,通过同步二维相关光谱图像结合残差卷积神经网络(ResNet)模型,可以成功区分两个不同层面的分类。训练集、测试集和外部验证的模型准确率均达到100%,交叉熵损失函数曲线较低。这证明了所提出的方法用于三七地理溯源的潜在可行性,即使采样点之间的距离很小。
本研究结果可以提高三七的质量,为未来三七的种植提供参考,并减少市场欺诈的发生。