Institute of Nanotechnology (INT) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Department of Materials, The University of Manchester, Manchester, UK.
Sci Rep. 2022 Mar 10;12(1):3895. doi: 10.1038/s41598-022-06225-4.
Biomedical applications such as cell screening or cell-cell interaction studies require placement and adhesion of cells on surfaces with controlled numbers and location. In particular, single-cell arraying and positioning has come into focus as a basis of such applications. An ideal substrate would combine biocompatibility with favorable attributes such as pattern stability and easy processing. Here, we present a simple yet effective approach to single-cell arraying based on a graphene oxide (GO) surface carrying protein (fibronectin) microarrays to define cell adhesion points. These capture NIH-3T3 cells, resulting in cell arrays, which are benchmarked against analogous arrays on silanized glass samples. We reveal significant improvement in cell-capture performance by the GO coating with regards to overall cell adhesion and single-cell feature occupancy. This overall improvement of cell-arraying combined with retained transparency of substrate for microscopy and good biocompatibility makes this graphene-based approach attractive for single-cell experiments.
生物医学应用，如细胞筛选或细胞-细胞相互作用研究，需要在具有受控数量和位置的表面上放置和黏附细胞。特别是，单细胞阵列和定位已经成为此类应用的基础。理想的基质应将生物相容性与图案稳定性和易于加工等有利属性结合起来。在这里，我们提出了一种简单而有效的单细胞阵列方法，该方法基于携带蛋白质（纤连蛋白）微阵列的氧化石墨烯（GO）表面来定义细胞黏附点。这些捕获 NIH-3T3 细胞，形成细胞阵列，并与硅烷化玻璃样品上的类似阵列进行基准测试。我们发现，与 GO 涂层相比，整体细胞黏附性和单细胞特征占有率都有显著提高。这种细胞阵列的整体改进与保留用于显微镜检查的基质的透明度和良好的生物相容性相结合，使得这种基于石墨烯的方法在单细胞实验中具有吸引力。
