Martinotti M G, Martinetto P, Savoia D
Eur J Clin Microbiol. 1986 Jun;5(3):320-3. doi: 10.1007/BF02017789.
The in vitro adherence to WISH cells of a pathogenic Trichomonas vaginalis strain was studied with a method utilizing thymidine-labeled protozoa. A marked dose-related adherence was observed. Glutaraldehyde fixed trichomonads were not adherent. The presence of fetal calf serum during the assay did not influence attachment. Concanavalin A inhibited adherence of protozoa. Complete or partial inhibition of adherence was achieved by preincubating WISH cells with Lactobacillus fermentum or Streptococcus agalactiae. Finally, pretreatment of cells with alpha-estradiol, beta-estradiol, progesterone and estrone influenced attachment of protozoa, whereas estriol was ineffective. These results suggest that adherence of Trichomonas vaginalis is dependent on different factors, whose manipulation may have clinical relevance in preventing recurrence of trichomonad vaginitis.
采用利用胸腺嘧啶核苷标记原生动物的方法，研究了一株致病性阴道毛滴虫对WISH细胞的体外黏附情况。观察到明显的剂量相关黏附现象。经戊二醛固定的滴虫不具有黏附性。检测过程中胎牛血清的存在不影响黏附。伴刀豆球蛋白A抑制原生动物的黏附。用发酵乳杆菌或无乳链球菌预孵育WISH细胞可实现对黏附的完全或部分抑制。最后，用α-雌二醇、β-雌二醇、孕酮和雌酮对细胞进行预处理会影响原生动物的黏附，而雌三醇则无效。这些结果表明，阴道毛滴虫的黏附取决于不同因素，对这些因素的操控在预防滴虫性阴道炎复发方面可能具有临床意义。
