Shanghai Key Laboratory of Brain Functional Genomics (Ministry of Education), Affiliated Mental Health Center (ECNU), School of Psychology and Cognitive Science, East China Normal University, Shanghai 200062, China.
NYU-ECNU Institute of Brain and Cognitive Science at NYU Shanghai, Shanghai 200062, China.
STAR Protoc. 2022 Mar 7;3(1):101221. doi: 10.1016/j.xpro.2022.101221. eCollection 2022 Mar 18.
Brain-region-specific drug infusion is a key way to investigate neural mechanisms underlying behavior and neurological diseases. Here, we present a detailed protocol for cannula implantation, intra-brain drug infusion, and two reward-seeking-related behavioral paradigms in mice: the light/dark box test and touchscreen version of progressive ratio test. In addition, we provide a user-friendly Python-based tool for behavioral data analysis. This protocol can be easily adapted to address various research questions related to behavioral pharmacology. For complete details on the use and execution of this protocol, please refer to Peng et al. (2021).
脑区特定药物输注是研究行为和神经疾病相关神经机制的重要方法。本文提供了详细的套管植入、脑内药物输注以及两种与奖赏寻求相关的小鼠行为范式（明暗箱测试和触屏式递增比率测试）的实验方案。此外，我们还提供了一个基于 Python 的简单易用的行为数据分析工具。该方案可轻松适用于解决与行为药理学相关的各种研究问题。如需了解本方案的详细使用和执行方法，请参考 Peng 等人（2021 年）的文献。
