Zuieva Anastasiia, Can Suzan, Boelke Franziska, Reuter Stefanie, Schattscheider Sebastian, Töpfer Elfi, Westphal Anika, Mrowka Ralf, Wölfl Stefan
Institute of Pharmacy and Molecular Biotechnology, Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany.
Microfluidic ChipShop GmbH, Jena, Germany, Stockholmer Str. 20, 07747, Jena, Germany.
Arch Toxicol. 2022 May;96(5):1483-1487. doi: 10.1007/s00204-022-03272-8. Epub 2022 Mar 19.
Microfluidic tissue culture and organ-on-a-chip models provide efficient tools for drug testing in vivo and are considered to become the basis of in vitro test systems to analyze drug response, drug interactions and toxicity to complement and reduce animal testing. A major limitation is the efficient recording of drug action. Here we present an efficient experimental setup that allows long-term cultivation of cells in a microfluidic system in combination with continuous recording of luciferase reporter gene expression. The system combines a sensitive cooled luminescence camera system in combination with a custom build miniaturized incubation chamber. The setup allows to monitor time-dependent activation, but also the end of drug response. Repeated activation and recovery as well as varying durations of drug treatment periods can be monitored, and different modes of drug activity can be visualized.
微流控组织培养和芯片器官模型为体内药物测试提供了高效工具，被认为将成为体外测试系统的基础，用于分析药物反应、药物相互作用和毒性，以补充和减少动物试验。一个主要限制是药物作用的有效记录。在此，我们展示了一种高效的实验装置，该装置允许在微流控系统中对细胞进行长期培养，并结合对荧光素酶报告基因表达的连续记录。该系统将一个灵敏的冷却发光相机系统与一个定制的小型化培养室相结合。该装置不仅可以监测随时间的激活情况，还能监测药物反应的结束。可以监测重复的激活和恢复以及不同持续时间的药物治疗期，并且可以可视化不同的药物活性模式。
