负责大肠杆菌中D-木糖摄取的基因的核苷酸序列。
Nucleotide sequence of the gene responsible for D-xylose uptake in Escherichia coli.
作者信息
Kurose N, Watanabe K, Kimura A
出版信息
Nucleic Acids Res. 1986 Sep 11;14(17):7115-23. doi: 10.1093/nar/14.17.7115.
The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature. Nucleotide sequence analysis found one possible reading frame coding for a polypeptide consisting of 61 amino acids. Several signal sequences conserved in the promoter regions of E. coli were found in the upstream regions of the open frame. This included the Shine-Dalgarno sequence, the Pribnow box, and the sequence conserved in the "-35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequence was present, followed by 3 successive T's.
已确定克隆的DNA的核苷酸序列，其长度为363 bp。它可以互补在低温下D-木糖摄取活性降低的大肠杆菌的突变。核苷酸序列分析发现一个可能的阅读框，编码一个由61个氨基酸组成的多肽。在开放阅读框的上游区域发现了大肠杆菌启动子区域中保守的几个信号序列。这包括Shine-Dalgarno序列、Pribnow框以及在“-35区域”中保守的序列，它们彼此之间具有合适的间距以实现高效转录。在终止密码子下游，存在反向重复序列，其后跟着3个连续的T。
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