Gaba Fortis, Tipping William J, Salji Mark, Faulds Karen, Graham Duncan, Leung Hing Y
Department of Urology, Queen Elizabeth University Hospital, NHS Greater Glasgow and Clyde, Glasgow G51 4TF, UK.
School of Medicine, University of Glasgow, Glasgow G12 8QQ, UK.
Cancers (Basel). 2022 Mar 17;14(6):1535. doi: 10.3390/cancers14061535.
Optical techniques are widely used tools in the visualisation of biological species within complex matrices, including biopsies, tissue resections and biofluids. Raman spectroscopy is an emerging analytical approach that probes the molecular signature of endogenous cellular biomolecules under biocompatible conditions with high spatial resolution. Applications of Raman spectroscopy in prostate cancer include biopsy analysis, assessment of surgical margins and monitoring of treatment efficacy. The advent of advanced Raman imaging techniques, such as stimulated Raman scattering, is creating opportunities for real-time in situ evaluation of prostate cancer. This review provides a focus on the recent preclinical and clinical achievements in implementing Raman-based techniques, highlighting remaining challenges for clinical applications. The research and clinical results achieved through in vivo and ex vivo Raman spectroscopy illustrate areas where these evolving technologies can be best translated into clinical practice.
光学技术是可视化复杂基质中生物物种的广泛使用的工具，这些复杂基质包括活检组织、组织切除术样本和生物流体。拉曼光谱是一种新兴的分析方法，可在生物相容条件下以高空间分辨率探测内源性细胞生物分子的分子特征。拉曼光谱在前列腺癌中的应用包括活检分析、手术切缘评估和治疗效果监测。先进的拉曼成像技术（如受激拉曼散射）的出现为前列腺癌的实时原位评估创造了机会。本综述重点关注基于拉曼技术的临床前和临床最新成果，突出临床应用中尚存的挑战。通过体内和体外拉曼光谱取得的研究和临床结果阐明了这些不断发展的技术最能转化为临床实践的领域。
