Centre for Inflammation Research, The University of, Edinburgh, UK.
Institute of Molecular Plant Sciences, The University of Edinburgh, UK.
Angew Chem Int Ed Engl. 2021 Mar 29;60(14):7637-7642. doi: 10.1002/anie.202016802. Epub 2021 Feb 26.
Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.
蔗糖是植物中用于长途运输的主要糖，在能量代谢中起着至关重要的作用；然而，目前还没有用于实时成像的活体细胞类似物。我们已经优化了一种合成方法来制备蔗糖类似物，包括在果糖部分中非常小的（≈50Da 或更小）拉曼标记物。光谱分析确定炔基标记化合物 6 为一种可被活细胞内源性转运体识别的蔗糖类似物，其拉曼强度高于其他蔗糖衍生物。在此，我们证明了使用化合物 6 作为第一个光学探针，通过拉曼显微镜实时观察活植物细胞中蔗糖的摄取和细胞内定位。
