Department of Chemistry, Emory University, Atlanta, GA, USA.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
Nat Nanotechnol. 2022 May;17(5):514-523. doi: 10.1038/s41565-022-01080-w. Epub 2022 Mar 28.
DNA has become the biomolecule of choice for molecular computation that may one day complement conventional silicon-based processors. In general, DNA computation is conducted in individual tubes, is slow in generating chemical outputs in response to chemical inputs and requires fluorescence readout. Here, we introduce a new paradigm for DNA computation where the chemical input is processed and transduced into a mechanical output using dynamic DNA-based motors operating far from equilibrium. We show that DNA-based motors with onboard logic (DMOLs) can perform Boolean functions (NOT, YES, AND and OR) with 15 min readout times. Because DMOLs are micrometre-sized, massive arrays of DMOLs that are identical or uniquely encoded by size and refractive index can be multiplexed and perform motor-to-motor communication on the same chip. Finally, DMOL computational outputs can be detected using a conventional smartphone camera, thus transducing chemical information into the electronic domain in a facile manner, suggesting potential applications.
DNA 已成为分子计算的首选生物分子，它可能有一天会补充传统的硅基处理器。一般来说，DNA 计算是在单独的试管中进行的，它在响应化学输入时生成化学输出的速度较慢，并且需要荧光读出。在这里，我们引入了一种新的 DNA 计算范式，其中使用远离平衡的基于 DNA 的动态马达将化学输入处理并转换为机械输出。我们表明，具有板载逻辑（DMOL）的基于 DNA 的马达可以在 15 分钟的读取时间内执行布尔函数（NOT、YES、AND 和 OR）。由于 DMOL 是微尺寸的，因此可以对相同或仅通过尺寸和折射率进行编码的大量 DMOL 阵列进行复用，并在同一芯片上进行马达到马达的通信。最后，DMOL 计算输出可以使用常规的智能手机摄像头进行检测，从而以简单的方式将化学信息转换为电子域，这表明了其潜在的应用。
