Department of Periodontology, School of Dentistry, Jeonbuk National University, Jeonju 54907, Korea.
Department of Periodontology, School of Dentistry, Beihua University, Jilin 132013, China.
Int J Environ Res Public Health. 2022 Mar 30;19(7):4113. doi: 10.3390/ijerph19074113.
Periodontitis is a chronic inflammatory condition characterized by gingival infection, periodontal pocket formation, and alveolar bone loss. Acidic mammalian chitinase (AMCase), an active chitinase enzyme, increased its expression under severe inflammation and related systemic disorders. However, AMCase expression and molecular mechanism in periodontal inflammation, have not been elucidated yet. This study was aimed to characterize AMCase in severe periodontitis patients compare to those in periodontally healthy subjects. In total, 15 periodontally healthy subjects and 15 severe (stage III/IV) periodontitis patients were enrolled with their informed consent. Tissue samples were collected and analyzed using Western blot and enzyme-linked immunosorbent assay (ELISA). AMCase protein expressions in periodontal patients were significantly more increased than those of periodontally healthy individuals. ELISA resulted in median values (first quartile to third quartile) of the periodontally healthy group 0.654 ng/mL (range, 0.644−0.827 ng/mL) and the periodontitis group 0.965 ng/mL (range, 0.886−1.165 ng/mL). AMCase was expressed significantly higher levels in periodontitis patients than in periodontally healthy individuals (p < 0.05). This suggests that AMCase may play a potential role as a biomarker for the screening and early diagnosis of severe periodontitis.
牙周炎是一种慢性炎症性疾病，其特征为牙龈感染、牙周袋形成和牙槽骨丧失。酸性哺乳动物几丁质酶（AMCase）是一种活性几丁质酶，在严重炎症和相关系统性疾病下表达增加。然而，AMCase 在牙周炎炎症中的表达和分子机制尚未阐明。本研究旨在表征严重牙周炎患者和牙周健康个体中的 AMCase。总共有 15 名牙周健康受试者和 15 名严重（III/IV 期）牙周炎患者在知情同意的情况下参与了研究。使用 Western blot 和酶联免疫吸附试验（ELISA）分析收集的组织样本。牙周炎患者的 AMCase 蛋白表达明显高于牙周健康个体。ELISA 的中位数（第一四分位数到第三四分位数）值为牙周健康组 0.654ng/mL（范围，0.644-0.827ng/mL）和牙周炎组 0.965ng/mL（范围，0.886-1.165ng/mL）。牙周炎患者的 AMCase 表达水平明显高于牙周健康个体（p<0.05）。这表明 AMCase 可能作为严重牙周炎筛查和早期诊断的潜在生物标志物。
