Bustos Cristina, Quezada Johan, Veas Rhonda, Altamirano Claudia, Braun-Galleani Stephanie, Fickers Patrick, Berrios Julio
School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso 2362803, Chile.
Microbial Processes and Interactions, TERRA Teaching and Research Centre, Gembloux Agro-Bio Tech, University of Liège, Av. de la Faculté 2B, 5030 Gembloux, Belgium.
Metabolites. 2022 Apr 14;12(4):346. doi: 10.3390/metabo12040346.
(formerly known as ) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
（以前称为 ）已成为重组蛋白生产中越来越重要的微生物。这种酵母菌种在工业环境中对生产多种蛋白质（包括酶和生物制药）引起了高度关注。在过去几十年中，为了提高重组蛋白的生产率和降低生产成本，已经取得了相关的生物工艺进展。最近，也考虑为此目的改善细胞特性和性能，并且已经报道了对蛋白生产率有直接和重大影响的有前景的策略。在这篇综述中，讨论了包括代谢工程和能量供应、转录因子调节以及重组蛋白折叠和分泌所涉及途径的操纵等细胞工程方法。还证明了在涉及培养参数优化的更高规模生物反应器中进行的研究不足，这突出了需要考虑的新研究目标。
