Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Department of Geriatric Dentistry, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, 100081, China.
Adv Mater. 2022 Jul;34(27):e2202180. doi: 10.1002/adma.202202180. Epub 2022 May 31.
Programmable base pair interactions at the nanoscale make DNA an attractive scaffold for forming hydroxyapatite (HAP) nanostructures. However, engineering macroscale HAP mineralization guided by DNA molecules remains challenging. To overcome this issue, a facile strategy is developed for the fabrication of ultrastiff DNA-HAP bulk composites. The electrostatic complexation of DNA and a surfactant with a quaternary ammonium salt group enables the formation of long-range ordered scaffolds using electrospinning. The growth of 1D and 2D HAP minerals is thus realized by this DNA template at a macroscale. Remarkably, the as-prepared DNA-HAP composites exhibit an ultrahigh Young's modulus of ≈25 GPa, which is comparable to natural HAP and superior to most artificial mineralized composites. Furthermore, a new type of dental inlay with outstanding antibacterial properties is developed using the stiff DNA-HAP. The encapsulated quaternary ammonium group within the dense HAP endows the composite with long-lasting and local antibacterial activity. Therefore, this new type of super-stiff biomaterial holds great potential for oral prosthetic applications.
在纳米尺度上可编程的碱基对相互作用使 DNA 成为形成羟基磷灰石（HAP）纳米结构的理想支架。然而，受 DNA 分子引导的宏观规模 HAP 矿化工程仍然具有挑战性。为了解决这个问题，开发了一种简便的策略来制造超硬 DNA-HAP 块状复合材料。DNA 和带季铵盐基团的表面活性剂的静电络合可以通过静电纺丝形成长程有序的支架。因此，通过这种 DNA 模板在宏观尺度上实现了 1D 和 2D HAP 矿物的生长。值得注意的是，所制备的 DNA-HAP 复合材料表现出超高的杨氏模量≈25 GPa，与天然 HAP 相当，优于大多数人工矿化复合材料。此外，还使用刚性 DNA-HAP 开发了一种具有出色抗菌性能的新型牙嵌体。致密 HAP 内包裹的季铵基团赋予复合材料持久且局部的抗菌活性。因此，这种新型超硬生物材料在口腔修复应用中具有很大的潜力。
