Center for Advanced Functional Nanorobots, Department of Inorganic Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology Prague, Technická 5, Prague, 166 28, Czech Republic.
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno, 61300, Czech Republic.
Small. 2022 Jun;18(23):e2200208. doi: 10.1002/smll.202200208. Epub 2022 May 10.
Micro/nanorobots represent a new generation of micromachines that can accomplish various tasks, such as loading and transporting specific targets or pharmaceuticals for a given application. Biohybrid robots consisting of biological cells (bacteria, sperm, and microalgae) combined with inorganic particles to control or propel their movement are of particular interest. The skeleton of these biohybrid robots can be used to load biomolecules. In this work, the authors create biohybrid robots based on tomato plants by coculturing ferromagnetic nanoparticles (Fe O ) with tomato callus cells. The tomato-based biohybrid robots (Tomato-Biobots) containing Fe O nanoparticles are driven by a transversely rotating magnetic field. In addition, biohybrid robots are used to load vitamin C, to generate clones of tomato cells. It is shown that the presence of Fe O does not affect the growth of tomato callus. This study opens a wide range of possibilities for the use of biohybrid robots@Fe O to deliver conventional agrochemicals, including fertilizers, pesticides, and herbicides, and allows for a gradual and sustained release of nutrients and agrochemicals, leading to precise dosing that reduces the amount of agrochemicals used. This conceptually new type of micromachine with application to plants and agronomy shall find broad use in this field.
微/纳米机器人代表了新一代的微型机器，可以完成各种任务，例如加载和运输特定的目标或药物用于给定的应用。由生物细胞（细菌、精子和微藻）与无机颗粒结合而成的生物混合机器人，用于控制或推动它们的运动，这引起了人们的特别关注。这些生物混合机器人的骨架可用于加载生物分子。在这项工作中，作者通过共培养与番茄愈伤组织细胞结合的铁氧体纳米颗粒（Fe3O4）来创建基于番茄的生物混合机器人（Tomato-Biobots）。基于番茄的生物混合机器人（Tomato-Biobots）含有 Fe3O4 纳米颗粒，可以在横向旋转磁场的作用下移动。此外，生物混合机器人还可以用于加载维生素 C，生成番茄细胞的克隆。结果表明，Fe3O4 的存在并不影响番茄愈伤组织的生长。这项研究为使用生物混合机器人@Fe3O4 来输送传统的农用化学品（包括肥料、农药和除草剂）开辟了广泛的可能性，并允许营养物质和农用化学品的逐渐和持续释放，从而实现精确的剂量控制，减少农用化学品的使用量。这种具有植物和农业应用的新概念型微型机器将在该领域得到广泛应用。
