Yang Tai, Liu Bifan, Wang Yujie, Huang Xiangying, Yan Zhaoming, Jiang Qian, Chen Qinghua
Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China.
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
Animals (Basel). 2022 May 4;12(9):1180. doi: 10.3390/ani12091180.
Heat stress (HS) has been revealed to damage the antioxidant system and intestinal barrier function, which greatly threatens poultry production. The present study investigated the effects of dietary ellagic acid (EA) on the antioxidant system, gut barrier function, and gut microbiota of heat-stressed broilers. Arbor Acres 14-day-old broilers numbering 360 were randomly divided into six groups, including one negative control group (NC) and five experimental groups. The broilers in the NC group were supplemented with a basal diet at a normal temperature (23 ± 2 °C). The broilers in the experimental groups were supplemented with basal diets containing EA at different doses (0, 75, 150, 300, and 600 mg/kg) at HS temperature (35 ± 2 °C). The experiment lasted for 4 weeks. Results showed that dietary EA reduced the corticosterone (CORT), LPS, and diamine oxidase (DAO) levels in the serum of heat-stressed broilers. Additionally, dietary EA improved the antioxidant enzyme activity and mRNA levels of Nrf2/HO-1 in the ileum of heat-stressed broilers. The relative abundances of , , , , , and in the cecum were significantly reduced by the EA supplementation in a dose-dependent manner. Notably, the LPS, DAO, and MDA in the serum were revealed to be positively correlated with the relative abundances of and , while the GSH-px, SOD, and CAT levels in the serum were negatively correlated with the relative abundances of and . Taken together, dietary EA improved the antioxidant capacity, intestinal barrier function, and alleviated heat-stressed injuries probably via regulating gut microbiota.
热应激（HS）已被证明会损害抗氧化系统和肠道屏障功能，这对家禽生产构成了极大威胁。本研究调查了日粮鞣花酸（EA）对热应激肉鸡抗氧化系统、肠道屏障功能和肠道微生物群的影响。将360只14日龄的爱拔益加肉鸡随机分为六组，包括一个阴性对照组（NC）和五个实验组。NC组的肉鸡在常温（23±2°C）下饲喂基础日粮。实验组的肉鸡在热应激温度（35±2°C）下饲喂含有不同剂量（0、75、150、300和600 mg/kg）EA的基础日粮。实验持续4周。结果表明，日粮EA降低了热应激肉鸡血清中的皮质酮（CORT）、脂多糖（LPS）和二胺氧化酶（DAO）水平。此外，日粮EA提高了热应激肉鸡回肠中抗氧化酶活性和Nrf2/HO-1的mRNA水平。添加EA后，盲肠中、、、、和的相对丰度呈剂量依赖性显著降低。值得注意的是，血清中的LPS、DAO和丙二醛（MDA）与和的相对丰度呈正相关，而血清中的谷胱甘肽过氧化物酶（GSH-px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平与和的相对丰度呈负相关。综上所述，日粮EA可能通过调节肠道微生物群提高了抗氧化能力、肠道屏障功能，并减轻了热应激损伤。
